Antiviral activity of luteolin against Japanese encephalitis virus
Antiviral activity of luteolin against Japanese encephalitis virus
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木犀草素对日本脑炎病毒的抗病毒活性
DOI：
10.1016/j.virusres.2016.04.021
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发表时间：
2016-07-15
期刊：
影响因子：
5
通讯作者：
Li, Xiangmin
中科院分区：
文献类型：
--
作者：
Fan, Wenchun;Qian, Suhong;Li, Xiangmin
日本脑炎病毒（JEV）属于黄病毒科，是一种嗜神经性黄病毒，可引起日本脑炎（JE）。乙脑病毒是人类病毒性脑炎最重要的病原体之一，这种疾病导致很高的死亡率。尽管已有有效的疫苗，但尚未开发出有效的乙脑抗病毒疗法。因此，寻找有效的抗乙脑病毒感染的抗病毒药物非常重要。在这项研究中，我们发现木犀草素是一种抗病毒生物类黄酮，对 A549 细胞中的 JEV 复制具有有效的抗病毒活性，IC50 = 4.56 mu g/mL。木犀草素还表现出对 JEV 的细胞外杀病毒活性。药物添加时间测定表明，JEV 复制在进入阶段后被木犀草素抑制。总体而言，我们的结果表明木犀草素可用于开发针对 JEV 的抗病毒药物。 (C) 2016 Elsevier B.V. 保留所有权利。
Japanese encephalitis virus (JEV), a member of family Flaviviridae, is a neurotropic flavivirus that causes Japanese encephalitis (JE). JEV is one of the most important causative agents of viral encephalitis in humans, and this disease leads to high fatality rates. Although effective vaccines are available, no effective antiviral therapy for JE has been developed. Hence, identifying effective antiviral agents against JEV infection is important. In this study, we found that luteolin was an antiviral bioflavonoid with potent antiviral activity against JEV replication in A549 cells with IC50 = 4.56 mu g/mL. Luteolin also showed extra cellular virucidal activity on JEV. With a time-of-drug addition assay revealing that JEV replication was inhibited by luteolin after the entry stage. Overall, our results suggested that luteolin can be used to develop an antiviral drug against JEV. (C) 2016 Elsevier B.V. All rights reserved.