MiR-424 and miR-155 deregulated expression in cytogenetically normal acute myeloid leukaemia: correlation with NPM1 and FLT3 mutation status.
MiR-424 and miR-155 deregulated expression in cytogenetically normal acute myeloid leukaemia: correlation with NPM1 and FLT3 mutation status.
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DOI：
10.1186/1756-8722-5-26
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发表时间：
2012-06-08
影响因子：
28.5
通讯作者：
Lo-Coco F
中科院分区：
文献类型：
--
作者：
Faraoni I;Laterza S;Ardiri D;Ciardi C;Fazi F;Lo-Coco F
MicroRNA在正常造血中起中心作用，在急性髓性白血病（AML）中不受调节。该研究的目的是通过qRT-PCR研究48例细胞遗传学正常的AML患者中参与髓系分化的mirna （miR-424, miR-155, miR-223, miR-17-5p）的表达，这些患者具有NPM1和/或FLT3突变的特征。三种类型的归一化用于数据验证。我们发现miR-424在具有NPM1mutA的aml中下调，无论FLT3状态如何。相反，miR-155在具有或不具有NPM1突变的FLT3内部串联重复（ITD）患者中表现出上调。通过分析miR-223和miR-17-5p与FLT3和NPM1状态的关系，未发现显著相关性。该研究支持了CN-AML的主要遗传亚群与不同的miRNA特征相关的观点，并表明miR-424和miR-155的失调分别参与了NPM1和FLT3-ITD突变的CN-AML的发病机制。
MicroRNA have a central role in normal haematopoiesis and are deregulated in acute myeloid leukaemia (AML). The purpose of the study was to investigate by qRT-PCR the expression of miRNAs involved in myeloid differentiation (miR-424, miR-155, miR-223, miR-17-5p) in 48 patients with cytogenetically normal AML well characterized for NPM1 and/or FLT3 mutations. Three types of normalization were used for the data validation. We found that miR-424 was down-modulated in AMLs with NPM1mutA regardless of FLT3 status. On the contrary, miR-155 showed up-regulation in patients with FLT3 internal tandem duplications (ITD) with or without NPM1 mutations. No significant associations were found by analyzing miR-223 and miR-17-5p in relation to FLT3 and NPM1 status. This study supports the view that major genetic subsets of CN-AML are associated with distinct miRNA signatures and suggests that miR-424 and miR-155 deregulation is involved in the pathogenesis of CN-AML with NPM1 and FLT3-ITD mutations, respectively.
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影响因子：
8.8
作者：
Havelange, V.;Garzon, R.;Croce, C. M.
通讯作者：
Croce, C. M.
影响因子：
28.5
作者：
Takahashi S
通讯作者：
Takahashi S
DOI：
10.1073/pnas.0706963104
发表时间：
2007-12-11
影响因子：
11.1
作者：
Rosa, A.;Ballarino, M.;Bozzoni, I.
通讯作者：
Bozzoni, I.
DOI：
10.3324/haematol.2011.046888
发表时间：
2011-12-01
期刊：
HAEMATOLOGICA-THE HEMATOLOGY JOURNAL
影响因子：
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作者：
Russ, Annika C.;Sander, Sandrine;Bullinger, Lars
通讯作者：
Bullinger, Lars
影响因子：
50.3
作者：
Fazi, Francesco;Racanicchi, Serena;Nervi, Clara
通讯作者：
Nervi, Clara