Production of chronic compression of the cauda equina in rats for use in studies of lumbar spinal canal stenosis
Production of chronic compression of the cauda equina in rats for use in studies of lumbar spinal canal stenosis
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DOI：
10.1097/00007632-199512150-00018
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发表时间：
1995-12-15
期刊：
影响因子：
3
通讯作者：
Yamano, Y
中科院分区：
文献类型：
--
作者：
Iwamoto, H;Kuwahara, H;Yamano, Y
研究设计。采用新模型对大鼠腰椎管狭窄的病理生理进行了分析。目的：对大鼠马尾进行慢性压迫，分析腰椎管狭窄的病理生理。背景资料摘要。迄今为止的压缩模型包括急性或亚急性压缩。慢性压迫的模型尚未建立。在3周龄大鼠L5处的脊柱周围固定一根不锈钢丝，并在椎体的腹侧插入一块钢板以保护其免受金属丝的伤害。随着老鼠的成长，金属丝切断了它们的椎管。术后1年出现椎管狭窄，无后肢瘫痪。矢状面狭窄占原始宽度的百分比为51.6±6.2%（平均±标准差）。在钢丝附近区域的组织学分析中，发现包括轴突变性，脱髓鞘，神经纤维空泡变性，硬膜内血管变窄，其数量减少。观察到头部和尾部到钢丝，硬膜内血管充血，这些充血血管附近的退行性灶，这些灶周围的纤维化，硬膜外血管充血，以及这些血管周围的纤维化。对6只狭窄大鼠进行电生理分析，发现马尾神经传导速度延迟。该动物模型的组织学和电生理结果与人类实验对象相似，因此该方法制备的大鼠可用于马尾慢性压迫的研究。
Study Design. The pathophysiology of lumbar spinal canal stenosis was analyzed with a new model in rats.Objectives. To produce chronic compression of the cauda equina in rats for analysis of the pathophysiology of lumbar spinal canal stenosis.Summary of Background Data. Models of compression to date have involved acute or subacute compression. A model of chronic compression has not been available.Methods. A stainless steel wire was fastened around the spine at L5 in 3-week-old rats, with a plate inserted against the ventral aspect of the vertebra to protect it from the wire. As the rats grew, the wire cut into the spinal canal. One year after the operation, canal stenosis was present without paralysis of the back limbs.Results. Stenosis seen in sagittal sections, as a percentage of the original width, was 51.6 +/- 6.2% (mean +/- standard deviation). In histologic analysis of the region near the wire, findings included axonal degeneration, demyelination, vacuolar degeneration of the nerve fibers, and narrowing of intradural blood vessels with a decrease in their number. Cephalad and caudad to the wire, congested intradural blood vessels, degenerative foci near these congested vessels, fibrosis around these foci, congested epidural vessels, and fibrosis around these vessels were observed. During electrophysiologic analysis (six rats with stenosis), the conduction velocity of the cauda equina was found to be delayed.Conclusions. The histologic and electrophysiologic findings in this animal model were similar to findings in human subjects, so rats prepared in this way should be useful for study of chronic compression of the cauda equina.