Triplex-forming oligonucleotides with unexpected affinity for a nontargeted GA repeat sequence
Triplex-forming oligonucleotides with unexpected affinity for a nontargeted GA repeat sequence
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DOI：
10.1089/oli.1.1997.7.125
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发表时间：
1997-04-01
期刊：
影响因子：
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通讯作者：
Rassart, E
中科院分区：
文献类型：
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作者：
Beaulieu, M;Barbeau, B;Rassart, E
我们检测了针对来自小鼠fl -1基因的嘌呤序列的两个部分的不同嘌呤odn形成三倍体的亲和力和特异性。正如预期的那样，与目标嘌呤链反平行的odn可以形成三倍DNA。我们通过ODN与序列中存在的GA重复的相互作用来解释这种不寻常的结合，我们进一步研究了该ODN与由14个GA重复组成的靶标的相互作用，出乎意料的是，一个ODN对部分互补的GA靶标的亲和力高于其完全互补的靶标。对于另一种ODN，与GA靶标的结合较弱，可能涉及以类似于交替链三倍体形成的方式跳过碱基。
We examined the affinity and the specificity of tripler formation for different purine ODNs directed against two portions of a purine sequence derived from the mouse fli-1 gene. As expected, the ODNs antiparallel to the purine strand of their target can form tripler DNA. One parallel ODN showed binding to its target sequence, We explain this unusual binding by an interaction of the ODN with a GA repetition present in the sequence, We further examined the interaction of this ODN with a target composed of 14 GA repetitions, Unexpectedly, one ODN shows higher affinity for a partially complementary GA target relative to its completely complementary target. For another ODN, the binding to the GA target is weaker and might involve skipping of bases in a way that resembles alternate strand tripler formation.