MULTI-TILLERING DWARF1, a new allele of BRITTLE CULM 12, affects plant height and tiller in rice
MULTI-TILLERING DWARF1, a new allele of BRITTLE CULM 12, affects plant height and tiller in rice
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DOI：
10.1007/s11434-015-0981-y
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发表时间：
2016-12
期刊：
影响因子：
18.9
通讯作者：
Haiping Yu;Deyong Ren;Yangzhou Zhu;Jiangmin Xu;Yuexing Wang;Ruifang Liu;Yunxia Fang;Zhenyuan Shi;Jiangjie Pan;Meiqin Lu;B. Ma;Jiang Hu;Yuchun Rao
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文献类型：
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作者：
Haiping Yu;Deyong Ren;Yangzhou Zhu;Jiangmin Xu;Yuexing Wang;Ruifang Liu;Yunxia Fang;Zhenyuan Shi;Jiangjie Pan;Meiqin Lu;B. Ma;Jiang Hu;Yuchun Rao
株高和分蘖是决定水稻株形和影响水稻产量的重要因素。本研究从甲基磺酸乙酯处理的粳稻品种武运粳7号中筛选到一个稳定的多分蘖矮秆突变体多分蘖矮秆1号（mtd 1）。与野生型相比，mtd 1突变体表现出多效性，包括矮化、分蘖多、脆秆和抽穗期延迟。采用图位克隆策略，将MTD 1基因定位于9号染色体短臂上约66 kb的区域。测序结果表明，mtd 1突变体中的LOC_Os09g02650（BC 12）基因发生了一个单核苷酸替换（G → A），导致翻译提前终止。MTD 1/BC 12编码序列的过表达挽救了mtd 1突变体的所有表型，包括株高和分蘖，证实BC 12是mtd 1突变体中的突变基因。定量逆转录PCR分析表明，MTD 1/BC 12能够负调控MONOCULM 1、IDEAL PLANT ARCHITECTURE 1和Tillering and Dwarf 1基因的表达，控制水稻分蘖。α-淀粉酶活性分析和赤霉素（GA）处理结果表明，突变体mtd 1的矮化表现依赖于GA的合成途径。这些结果有助于进一步揭示水稻生长发育的分子机制。
Plant height and tillering are crucial factors determining rice plant architecture and influencing rice grain production. In this study, multi-tillering dwarf1 (mtd1), a stable multi-tiller and dwarf mutant, was screened from the ethylmethane sulfonate-treated japonica rice variety Wuyunging7. Compared with the wild type, mtd1 mutant exhibited pleiotropic phenotypes, including dwarfism, more tillers, brittle culms and delayed heading date. By employing map-based cloning strategy, the gene MTD1 was finally mapped to an approximately 66-kb region on the short arm of chromosome 9. Sequencing results showed that the gene LOC_Os09g02650 (BC12) in mtd1 mutant had a single nucleotide substitution (G to A), which generated a premature translation stop. Over-expressing MTD1/BC12 coding sequence rescued all the phenotypes of mtd1 mutants including plant height and tillers, which confirms that BC12 is the mutated gene in mtd1 mutant. Quantitative reverse transcription-PCR analysis showed that MTD1/BC12 could negatively regulate the expression of MONOCULM 1, IDEAL PLANT ARCHITECTURE1 and Tillering and Dwarf 1, and control rice tillering. Remarkably, α-amylase activity analysis and gibberellic acid (GA) treatment showed that the dwarf phenotype of mtd1 mutant was dependent on GA biosynthesis pathway. These results facilitated to further uncover the molecular mechanism of the growth and development in rice.