Six epitopes reacting with human cytotoxic CD8+ T cells in the central region of the HIV-1 NEF protein.
Six epitopes reacting with human cytotoxic CD8+ T cells in the central region of the HIV-1 NEF protein.
复制标题
HIV-1 NEF 蛋白中心区域的六个表位与人类细胞毒性 CD8 T 细胞发生反应。
作者：
B. Culmann;E. Gomard;M. Kieny;B. Guy;F. Dreyfus;A. Saimot;D. Séréni;D. Sicard;J. Levy
为了鉴定HIV-1的NEF蛋白中特异性CTL识别的靶表位，用从HIV血清阳性供体产生的NEF特异性CTL测试了33个衍生自HIV-BRU序列的肽。结果表明：（1）它们均位于NEF蛋白质中部的两个区域，分别对应于第73 ~ 94位和第113 ~ 147位残基; 2)从单个供体产生的CTL可以识别NEF蛋白的几个肽。3)这些肽中的一些可以与至少两种或三种不同的HLA I类分子结合识别。4)两个不同的重叠表位存在于15个氨基酸的相对较短的序列中。这些结果表明，对应于不同HLA限制的多个表位可以共存于NEF蛋白的相对小的区域中。这些结果在疫苗策略中使用合成肽轴承CTL表位的影响进行了讨论。
In order to identify the target epitopes recognized by specific CTL in the NEF protein of HIV-1, 33 peptides derived from the HIV-BRU sequence were tested with NEF-specific CTL generated from HIV-seropositive donors. Six different epitopes were identified and several points were remarkable: 1) They were all located in two regions of the central part of the NEF protein corresponding to residues 73 to 94 and 113 to 147, respectively. 2) The CTL issued from a single donor could recognize several peptides of the NEF protein. 3) Some of these peptides could be recognized in association with at least two or three different HLA class I molecules. 4) Two different overlapping epitopes were present in a relatively short sequence of 15 amino acids. These results suggest that multiple epitopes corresponding to different HLA restrictions could coexist in a relatively small region of the NEF protein. The implications of these results in vaccine strategies using synthetic peptides bearing CTL epitopes are discussed.