Quantification of DNA/SWCNT Solvation Differences by Aqueous Two-Phase Separation.
Quantification of DNA/SWCNT Solvation Differences by Aqueous Two-Phase Separation.
复制标题
通过水相两相分离定量 DNA/SWCNT 溶剂化差异。
作者：
Yoona Yang;Akshaya Shankar;Thibault Aryaksama;M. Zheng;A. Jagota
利用双水相体系(ATP)可以有效地将单链DNA包裹的单壁碳纳米管(SWCNTs)分离成各种手性结构。分配是由两相之间混合的溶解特性的微小差异所驱动的。因此，除了作为一种分离技术，ATP系统还潜在地提供了一种量化和排序溶质(这里是DNA/SWCNT杂化材料)的溶解性质的方法，如溶剂化自由能和溶解度。在这项研究中，我们提出了两种不同的方法来定量分析DNA/SWCNT杂交物的ATP分配。首先，我们提出了一个利用相对于标准态的展开来提取各种DNA/SWCNT杂化的相对溶剂化自由能的模型。其次，通过分析杂化产物在ATP体系中的分配情况，提取了一个溶解度参数。这两种方法被发现是一致的，为每种方法都提供了一些信心，作为一种量化不同DNA/SWCNT杂交物的溶解度差异的方法。
Single-walled carbon nanotubes (SWCNTs) coated with single-stranded DNA can be effectively separated into various chiralities using an aqueous two-phase (ATP) system. Partitioning is driven by small differences in the dissolution characteristics of the hybrid between the two phases. Thus, in addition to being a separation technique, the ATP system potentially also offers a way to quantify and rank the dissolution properties of the solute (here the DNA/SWCNT hybrids), such as the solvation free energy and solubility. In this study, we propose two different approaches to quantitatively analyze the ATP partitioning of DNA/SWCNT hybrids. First, we present a model that extracts the relative solvation free energy of various DNA/SWCNT hybrids by using an expansion relative to a standard state. Second, we extract a solubility parameter by analyzing the partitioning of hybrids in the ATP system. The two approaches are found to be consistent, providing some confidence in each as a method of quantifying differences in the solubility of various DNA/SWCNT hybrids.