An integrated hybrid interference and absorption filter for fluorescence detection in lab-on-a-chip devices
An integrated hybrid interference and absorption filter for fluorescence detection in lab-on-a-chip devices
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DOI：
10.1039/b819080a
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发表时间：
2009-05-21
期刊：
影响因子：
6.1
通讯作者：
Charette, Paul G.
中科院分区：
文献类型：
--
作者：
Richard, Charles;Renaudin, Alan;Charette, Paul G.
我们提出了一种混合光学滤波器设计，它结合了干涉和吸收组件，用于增强小型化高度集成的芯片实验室设备中的荧光检测。滤波器的设计方式是利用每种技术的优点来抵消另一种技术的缺点。该过滤器是用微加工兼容工艺和材料制造的，用于与微电子和微流体装置单片集成。本文所述的滤光器的特定实施例被设计成区分650 nm处的荧光发射与532 nm处的激发。9层干涉滤光器组件由交替的TiO 2和SiO2薄膜层制成，并且在532 nm处具有-12.6 dB的全部衰减，在650 nm处具有-0.76 dB的全部衰减。使用在532 nm处具有-32.6 dB和在650 nm处具有-1.28 dB的所有衰减的染色光聚合物（KMPR + Orasol Red）制造吸收滤光器组件。该混合滤波器的总抑制比为43 dB。该滤波器表现出非常低的自发荧光，并在离轴入射角下同样表现良好。
We present a hybrid optical filter design that combines interference and absorbing components For enhanced fluorescence detection in miniaturized highly-integrated lab-on-a-chip devices. The filter is designed in such a way that the advantages of each technology are used to offset the disadvantages of the other. The filter is fabricated with microfabrication compatible processes and materials for monolithic integration with microelectronics and microfluidics devices. The particular embodiment of the filter described herein is designed to discriminate fluorescence emission at 650 nm from excitation at 532 rim. The 9-layer interference filter component is fabricated with alternating TiO2 and SiO2 thin-film layers and has all attenuation of -12.6 dB at 532 nm, and -0.76 dB at 650 inn. The absorbing filter component is fabricated using a dyed photopolymer (KMPR + Orasol Red) having all attenuation of -32.6 dB at 532 nm and -1.28 dB at 650 nm. The total rejection ratio of the hybrid filter is 43 dB. The filter exhibits very low autofluorescence and performs equally well at off-axis incidence angles.