Controlled Logic GatesSwitch Gate and Fredkin Gate Based on Enzyme-Biocatalyzed Reactions Realized in Flow Cells
Controlled Logic GatesSwitch Gate and Fredkin Gate Based on Enzyme-Biocatalyzed Reactions Realized in Flow Cells
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DOI：
10.1002/cphc.201501095
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发表时间：
2016-04-04
期刊：
影响因子：
2.9
通讯作者：
Katz, Evgeny
中科院分区：
文献类型：
--
作者：
Fratto, Brian E.;Katz, Evgeny
受控逻辑门，其中数据输入上的逻辑操作以控制信号确定的方式执行，以化学方式设计。具体地，已经基于在多池流动系统中进行的酶生物催化反应设计了其中根据控制输入信号的逻辑值将数据输出信号引导到各种输出通道的系统。在开关门中，根据控制输入信号的逻辑值，一个数据信号被引导到两个可能的输出通道之一。在可逆Fredkin门中，两个输出通道之间的两个数据信号的路由由第三控制信号控制。流动装置是使用流动池网络创建的，每个流动池用一种酶修饰，该酶生物催化一种化学反应。酶级联是通过将溶液从一个反应细胞移动到另一个反应细胞来实现的，这些反应细胞组织在一个特定的网络中。在流动装置中实现的基于酶的系统的模块化设计允许逻辑系统的容易重新配置，从而允许逻辑操作从开关门中的2输入/3输出通道简单扩展到Fredkin门中的3输入/3输出通道。通过使用流动池模块化设计，可以进一步增加用于实现各种逻辑过程的系统复杂性。
Controlled logic gates, where the logic operations on the Data inputs are performed in the way determined by the Control signal, were designed in a chemical fashion. Specifically, the systems where the Data output signals directed to various output channels depending on the logic value of the Control input signal have been designed based on enzyme biocatalyzed reactions performed in a multi-cell flow system. In the Switch gate one Data signal was directed to one of two possible output channels depending on the logic value of the Control input signal. In the reversible Fredkin gate the routing of two Data signals between two output channels is controlled by the third Control signal. The flow devices were created using a network of flow cells, each modified with one enzyme that biocatalyzed one chemical reaction. The enzymatic cascade was realized by moving the solution from one reacting cell to another which were organized in a specific network. The modular design of the enzyme-based systems realized in the flow device allowed easy reconfiguration of the logic system, thus allowing simple extension of the logic operation from the 2-input/3-output channels in the Switch gate to the 3-input/3-output channels in the Fredkin gate. Further increase of the system complexity for realization of various logic processes is feasible with the use of the flow cell modular design.