SWITCH TRANSCRIPTS IN IMMUNOGLOBULIN CLASS SWITCHING
SWITCH TRANSCRIPTS IN IMMUNOGLOBULIN CLASS SWITCHING
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DOI：
10.1126/science.7892607
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发表时间：
1995-03-24
期刊：
影响因子：
56.9
通讯作者：
RADBRUCH, A
中科院分区：
文献类型：
--
作者：
LORENZ, M;JUNG, S;RADBRUCH, A
B细胞可以交换免疫球蛋白重链恒定区域的基因片段，改变它们产生的抗体的类别和效应功能。类转换由细胞因子引导到不同的类，细胞因子诱导目标DNA序列的转录。这些转录本经过处理，产生剪接的“开关”转录本。在突变小鼠中，通过异源人金属硫蛋白IIA启动子可以将开关重组指向免疫球蛋白G1 （IgG1）。诱导结构保守的剪接开关转录物足以将开关重组靶向到IgG1，而仅靠转录是不够的。
B cells can exchange gene segments for the constant region of the immunoglobulin heavy chain, altering the class and effector function of the antibodies that they produce. Class switching is directed to distinct classes by cytokines, which induce transcription of the targeted DNA sequences. These transcripts are processed, resulting in spliced ''switch'' transcripts. Switch recombination can be directed to immunoglobulin G1 (IgG1) by the heterologous human metallothionein IIA promoter in mutant mice. Induction of the structurally conserved, spliced switch transcripts is sufficient to target switch recombination to IgG1, whereas transcription alone is not.