AtCHIP, a U-Box-Containing E3 Ubiquitin Ligase, Plays a Critical Role in Temperature Stress Tolerance in Arabidopsis1
AtCHIP, a U-Box-Containing E3 Ubiquitin Ligase, Plays a Critical Role in Temperature Stress Tolerance in Arabidopsis1
复制标题
DOI：
10.1104/pp.103.020800
复制
发表时间：
2003-06
期刊：
影响因子：
7.4
通讯作者：
Juqiang Yan;Jing Wang;Qingtian Li;J. Hwang;C. Patterson;Hong Zhang
中科院分区：
文献类型：
--
作者：
Juqiang Yan;Jing Wang;Qingtian Li;J. Hwang;C. Patterson;Hong Zhang
拟南芥AtCHIP基因编码的蛋白具有三个三肽重复序列和一个U盒结构域，其结构类似于动物CHIP蛋白，是一类新的E3泛素连接酶。与动物CHIP蛋白一样，AtCHIP在体外具有E3泛素连接酶活性。AtCHIP是一个单拷贝基因，其转录水平在低温和高温等逆境条件下上调。然而，单独增加AtCHIP表达与增加胁迫耐受性无关;事实上，AtCHIP在拟南芥中的过表达使植物对低温和高温处理更敏感。较高的电解质渗漏观察到AtCHIP过表达植物叶片低温处理后，表明膜功能可能会受损，在这些植物在这样的条件下。这些结果表明，AtCHIP在温度胁迫条件下植物细胞代谢中起着重要作用。
The Arabidopsis gene AtCHIP encodes a protein with three tetratricopeptide repeats and a U-box domain, which is structurally similar to the animal CHIP proteins, a new class of E3 ubiquitin ligases. Like animal CHIP proteins, AtCHIP has E3 ubiquitin ligase activity in vitro. AtCHIP is a single-copy gene, and its transcript is up-regulated by several stress conditions such as low and high temperatures. However, increased AtCHIP expression alone was not correlated with increased stress tolerance; in fact, overexpression of AtCHIP in Arabidopsis rendered plants more sensitive to both low- and high-temperature treatments. Higher electrolyte leakage was observed in leaves of AtCHIP overexpression plants after chilling temperature treatment, suggesting that membrane function is likely impaired in these plants under such a condition. These results indicate that AtCHIP plays an important role in plant cellular metabolism under temperature stress conditions.