The Synthetic Genome Summer Course.
The Synthetic Genome Summer Course.
复制标题
合成基因组暑期课程。
DOI：
10.1093/synbio/ysy020
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
Blount BA
中科院分区：
文献类型：
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作者：
Blount BA
举办合成基因组暑期课程的目的是向广大研究人员传授合成生物学和合成基因组科学最新进展背后的理论和实践技能，重点关注合成酵母基因组项目 Sc2.0。通过该领域领先成员的软件研讨会、教程和研究讲座，30 名与会者了解了相关原理和技术，然后他们能够在基于实验室的实践课程中亲自实施这些原理和技术。参与者对半合成酵母菌株进行 SCRaMbLEd 以使异源途径多样化，使用自动化来构建组合途径文库，并使用 CRISPR 来调试由合成染色体设计更改引起的适应性缺陷。探讨了合成染色体的社会影响，工业利益相关者从商业角度讨论了合成生物学。在 5 天的时间里，参与者获得了宝贵的见解并获得了帮助他们进行未来合成基因组研究的技能。
The Synthetic Genome Summer Course was convened with the aim of teaching a wide range of researchers the theory and practical skills behind recent advances in synthetic biology and synthetic genome science, with a focus on Sc2.0, the synthetic yeast genome project. Through software workshops, tutorials and research talks from leading members of the field, the 30 attendees learnt about relevant principles and techniques that they were then able to implement first-hand in laboratory-based practical sessions. Participants SCRaMbLEd semi-synthetic yeast strains to diversify heterologous pathways, used automation to build combinatorial pathway libraries and used CRISPR to debug fitness defects caused by synthetic chromosome design changes. Societal implications of synthetic chromosomes were explored and industrial stakeholders discussed synthetic biology from a commercial standpoint. Over the 5 days, participants gained valuable insight and acquired skills to aid them in future synthetic genome research.