Facile synthesis of magnetic metal organic frameworks for the enrichment of low‐abundance peptides for MALDI‐TOF MS analysis
Facile synthesis of magnetic metal organic frameworks for the enrichment of low‐abundance peptides for MALDI‐TOF MS analysis
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DOI：
10.1002/pmic.201300131
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发表时间：
2013-12
期刊：
影响因子：
3.4
通讯作者：
Man Zhao;Chunhui Deng;Xiangmin Zhang;Pengyuan Yang
中科院分区：
文献类型：
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作者：
Man Zhao;Chunhui Deng;Xiangmin Zhang;Pengyuan Yang
在这项工作中，通过用巯基乙酸涂覆磁铁矿颗粒并随后与Cu(OAc)2和苯-1,3,5-三羧酸（命名为H3btc）的乙醇溶液交替反应，成功合成了核壳磁性金属有机骨架（MOF）微球。所得的Fe3O4@[Cu3(btc)2]具有很强的磁响应性。我们首次将新型纳米复合材料与 MALDI-TOF MS 分析相结合，应用于富集低浓度标准肽、MYO 和 BSA 胰蛋白酶消化物以及人尿液中的肽。此外，Cu3(btc)2 MOF壳对肽表现出很强的亲和力，从而为低丰度肽的浓缩提供了一种快速便捷的方法。值得注意的是，在用磁性 MOF 复合材料富集后，MALDI-TOF MS 可以检测到 10 pM 的极低浓度的肽。简而言之，Fe3O4@[Cu3(btc)2]微球的简便合成和高效富集过程使其成为在复杂生物环境中分离肽的有希望的候选者。
In this work, core‐shell magnetic metal organic framework (MOF) microspheres were successfully synthesized by coating magnetite particles with mercaptoacetic acid and subsequent reactions with ethanol solutions of Cu(OAc)2 and benzene‐1,3,5‐tricarboxylic acid (designated as H3btc) alternately. The resulting Fe3O4@[Cu3(btc)2] possess strong magnetic responsiveness. We applied the novel nanocomposites in the enrichment of low‐concentration standard peptides, peptides in MYO and BSA tryptic digests and in human urine in combination with MALDI‐TOF MS analysis for the first time. In addition, the Cu3(btc)2 MOF shells exhibit strong affinity to peptides, thus providing a rapid and convenient approach to the concentration of low‐abundance peptides. Notably, peptides at an extremely low concentration of 10 pM could be detected by MALDI‐TOF MS after enrichment with the magnetic MOF composites. In brief, the facile synthesis and efficient enrichment process of the Fe3O4@[Cu3(btc)2] microspheres make them promising candidates for the isolation of peptides in even complex biological environments.