Different MicroRNA Families Involved in Regulating High Temperature Stress Response during Cotton (Gossypium hirsutum L.) Anther Development
Different MicroRNA Families Involved in Regulating High Temperature Stress Response during Cotton (Gossypium hirsutum L.) Anther Development
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不同 MicroRNA 家族参与调节棉花 (Gossypium hirsutum L.) 花药发育过程中的高温应激反应
DOI：
10.3390/ijms21041280
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发表时间：
2020-02-01
影响因子：
5.6
通讯作者：
Liu, Zhi
中科院分区：
文献类型：
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作者：
Chen, Jin;Pan, Ao;Liu, Zhi
MicroRNA（miRNAs）是一类广泛参与植物非生物胁迫应答的小分子RNA。我们对正常和高温（NT和HT）条件下棉花花药发育的4个阶段进行了小RNA测序，以研究miRNA对HT的胁迫响应特性。共鉴定出77种miRNA，其中包括33种已知miRNA和44种新型miRNA，其中NT和HT应激条件下分别有41种和28种miRNA差异表达。去除NT条件下基因型特异性miRNA的变化后，在造孢细胞增殖期（SCP）、减数分裂期（MP）、小孢子释放期（MRP）和花粉成熟期（PM）分别鉴定出10个（包括12个miRNA）、4个（包括6个miRNA）、4个（包括5个miRNA）和7个（包括11个miRNA）HT胁迫响应miRNA家族。7个miRNA家族（SCP期的miR 2949、miR 167和miR 160; MP期的miR 156和miR 172; MRP期的miR 156; PM期的miR 393和miR 3476）的表达丰度超过总表达丰度的10%，作为响应HT胁迫的主要调控因子，具有正或负调控模式。这些miRNAs协调了相应靶基因的表达，并导致HT耐受和HT敏感株系的不同反应。结果表明，棉花花药中miRNAs对高温胁迫的响应具有阶段特异性，且随花药发育的不同而不同。本研究为深入了解棉花花药中miRNAs对高温胁迫的响应及阐明植物对高温胁迫的耐受机制奠定了基础。
MicroRNAs (miRNAs) are small molecule RNAs widely involved in responses to plant abiotic stresses. We performed small RNA sequencing of cotton anthers at four developmental stages under normal and high temperature (NT and HT, respectively) conditions to investigate the stress response characteristics of miRNA to HT. A total of 77 miRNAs, including 33 known miRNAs and 44 novel miRNAs, were identified, and 41 and 28 miRNAs were differentially expressed under NT and HT stress conditions, respectively. The sporogenous cell proliferation (SCP), meiotic phase (MP), microspore release period (MRP), and pollen maturity (PM) stages had 10 (including 12 miRNAs), four (including six miRNAs), four (including five miRNAs), and seven (including 11 miRNAs) HT stress-responsive miRNA families, respectively, which were identified after removing the changes in genotype-specific miRNAs under NT condition. Seven miRNA families (miR2949, miR167, and miR160 at the SCP stage; miR156 and miR172 at the MP stage; miR156 at the MRP stage; and miR393 and miR3476 at the PM stage), which had expression abundance of more than 10% of the total expression abundance, served as the main regulators responding to HT stress with positive or negative regulation patterns. These miRNAs orchestrated the expression of the corresponding target genes and led to different responses in the HT-tolerant and the HT-sensitive lines. The results revealed that the HT stress response of miRNAs in cotton anthers were stage-specific and differed with the development of anthers. Our study may enhance the understanding of the response of miRNAs to HT stress in cotton anthers and may clarify the mechanism of plant tolerance to HT stress.