Lipidomic Profiling of Virus Infection Identifies Mediators that Resolve Herpes Simplex Virus-Induced Corneal Inflammatory Lesions
Lipidomic Profiling of Virus Infection Identifies Mediators that Resolve Herpes Simplex Virus-Induced Corneal Inflammatory Lesions
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病毒感染的脂质组学分析确定了解决单纯疱疹病毒引起的角膜炎症病变的介质
作者：
Cuiping Zhang;Zuojian Hu;Ke Wang;Lujie Yang;Yue Ni;Hartmut Schlüter;Pengyuan Yang;Jiaxu Hong;Hongxiu Yu
脂质介质（LMs）在炎症的诱导和消退中起着关键作用。为了确定和阐明它们在病毒感染过程中的参与，本研究对62种脂质进行了基于多反应监测（MRM）的液相色谱-串联质谱脂质组学分析。结果表明，RAW264.7巨噬细胞根据不同的病毒致病性差异产生特异性LMs信号。大规模脂质组学与靶向基因表达数据的整合揭示了介质，如RVD 3，18-HEPE，11（12）-EET等与感染的致病阶段相关。单纯疱疹病毒（HSV）诱导的角膜炎模型表明，11（12）-EET治疗代表了治疗病毒感染的新选择。
Lipid mediators（LMs）play a pivotal role in the induction and resolution of inflammation. To identify and elucidate their involvement during virus infection,.Multiple Reaction Monitoring (MRM) based liquid chromatography -tandem mass spectrometry lipidomic profiling of 62 lipid species was performed in this study. Results show that RAW264.7 macrophages differentially produce specific LMs signals depending on different virus pathogenicity. Integration of the large-scale lipidomics with targeted gene expression data revealed mediators, such as RVD3,18-HEPE, 11(12)-EET et al. correlated with the pathogenic phase of the infection. The Herpes Simplex Virus (HSV)-induced keratitis model demonstrates that 11(12)-EET treatment represents a novel alternative for treating viral infection.