Antiatherosclerotic Phenotype of Perivascular Adipose Tissue Surrounding the Saphenous Vein in Coronary Artery Bypass Grafting.
Antiatherosclerotic Phenotype of Perivascular Adipose Tissue Surrounding the Saphenous Vein in Coronary Artery Bypass Grafting.
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DOI：
10.1161/jaha.120.018905
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发表时间：
2021-04-06
影响因子：
5.4
通讯作者：
Kawaharada N
中科院分区：
文献类型：
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作者：
Mikami T;Furuhashi M;Sakai A;Numaguchi R;Harada R;Naraoka S;Kamada T;Higashiura Y;Tanaka M;Ohori S;Sakurada T;Nakamura M;Iba Y;Fukada J;Miura T;Kawaharada N
血管周围脂肪组织（PVAT）与代谢驱动的慢性炎症（称为后炎症）相关，后炎症有助于血管功能和血管疾病的发病机制。隐静脉（SV）通常用作冠状动脉旁路移植术中的重要管道，但SV移植物的长期通畅性是一个关键问题。据报道，使用SV及其周围组织的新型“无接触”采集技术可使SV移植物的长期通畅性良好。在此，我们研究了SV周围PVAT（SV-PVAT）与其他血管PVAT相比是否具有不同的表型。从48例接受择期冠状动脉旁路移植术的冠心病患者（男/女，32/16;年龄，72±8岁）中采集脂肪垫样本。SV-PVAT中的脂肪细胞尺寸显著大于胸廓内动脉、冠状动脉和主动脉周围PVAT中的尺寸。与冠状动脉和主动脉周围的PVAT相比，胸廓内动脉周围的SV-PVAT和PVAT的纤维化程度更小，纤维化相关标志物的基因表达水平降低，并且炎症反应更少，如分化簇11 c阳性M1巨噬细胞浸润的程度显著更小，脂联素的基因表达水平更高，炎性细胞因子的基因表达水平更低所示。脂肪细胞发育和模式形成基因的表达模式在血管的PVAT中完全不同。SV-PVAT的表型可能由脂肪细胞的固有差异引起，与冠状动脉周围的PVAT或主动脉周围的PVAT相比，SV-PVAT的表型更接近胸廓内动脉周围的PVAT。SV‐PVAT的后炎症和连续脂肪组织重塑较少，当使用SV采集的非接触技术时，这可能有助于移植物的长期通畅性。
Perivascular adipose tissue (PVAT) is associated with metabolically driven chronic inflammation called metaflammation, which contributes to vascular function and the pathogenesis of vascular disease. The saphenous vein (SV) is commonly used as an essential conduit in coronary artery bypass grafting, but the long‐term patency of SV grafts is a crucial issue. The use of the novel “no‐touch” technique of SV harvesting together with its surrounding tissue has been reported to result in good long‑term graft patency of SV grafts. Herein, we investigated whether PVAT surrounding the SV (SV‐PVAT) has distinct phenotypes compared with other PVATs of vessels. Fat pads were sampled from 48 patients (male/female, 32/16; age, 72±8 years) with coronary artery disease who underwent elective coronary artery bypass grafting. Adipocyte size in SV‐PVAT was significantly larger than the sizes in PVATs surrounding the internal thoracic artery, coronary artery, and aorta. SV‐PVAT and PVAT surrounding the internal thoracic artery had smaller extents of fibrosis, decreased gene expression levels of fibrosis‐related markers, and less metaflammation, as indicated by a significantly smaller extent of cluster of differentiation 11c–positive M1 macrophage infiltration, higher gene expression level of adiponectin, and lower gene expression levels of inflammatory cytokines, than did PVATs surrounding the coronary artery and aorta. Expression patterns of adipocyte developmental and pattern‐forming genes were totally different among the PVATs of the vessels. The phenotype of SV‐PVAT, which may result from inherent differences in adipocytes, is closer to that of PVAT surrounding the internal thoracic artery than that of PVAT surrounding the coronary artery or that of PVAT surrounding the aorta. SV‐PVAT has less metaflammation and consecutive adipose tissue remodeling, which may contribute to high long‐term patency of grafting when the no‐touch technique of SV harvesting is used.