Comparative analysis of Cryptococcus neoformans acid-resistant particles generated from pigmented cells grown in different laccase substrates
Comparative analysis of Cryptococcus neoformans acid-resistant particles generated from pigmented cells grown in different laccase substrates
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DOI：
10.1016/j.fgb.2005.09.003
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发表时间：
2005-12-01
影响因子：
3
通讯作者：
Casadevall, A
中科院分区：
文献类型：
--
作者：
Garcia-Rivera, J;Eisenman, HC;Casadevall, A
新生隐球菌在外源性底物存在下体外产生色素。我们的特点是耐酸颗粒分离色素细胞生长在左旋多巴，甲基多巴，（-）-肾上腺素或（-）-去甲肾上腺素。本研究的目的是确定由这些底物中的每一种制成的色素是否是黑色素以及色素沉着对相关细胞特征的影响。最大产率的耐酸颗粒发生与甲基多巴，其次是L-多巴。电子显微镜观察表明，左旋多巴和甲基多巴产生的颗粒具有较厚的外壳。mAb 6D2与所有颗粒反应，但与肾上腺素衍生颗粒观察到较低的反应性。ESR分析表明，肾上腺素衍生的颗粒未能产生稳定的自由基信号典型的黑色素。新生隐球菌在不同基质中的生长影响细胞和荚膜大小，但不影响荚膜诱导。因此，新型隐球菌产生的色素类型取决于底物，并非所有色素都符合黑色素的标准。(c)2005年爱思唯尔公司All rights reserved.
Cryptococcus neoformans produces pigments in vitro in the presence of exogenous substrate. We characterized acid-resistant particles isolated from pigmented cells grown in L-dopa, methyl-dopa, (-)-epinephrine or (-)-norepinephrine. The goals of this study were to determine whether pigments made from each of these substrates were melanins and the consequences of pigmentation on related cell characteristics. The greatest yield of acid-resistant particles occurred with methyl-dopa followed by L-dopa. Electron microscopy indicated that L-dopa and methyl-dopa produced particles with thicker shells. The mAb 6D2 reacted with all particles, but a lower reactivity was observed with epinephrine-derived particles. ESR analysis revealed that epinephrine-derived particles failed to produce a stable free radical signal typical of melanins. Growth of C neoformans in different substrates affected cell and capsule size but not capsule induction. Hence, the type of pigment produced by C neoformans is dependent on the substrate and not all pigments meet the criteria for melanins. (c) 2005 Elsevier Inc. All rights reserved.