Depth-resolved multimodal imaging: Wavelength modulated spatially offset Raman spectroscopy with optical coherence tomography
Depth-resolved multimodal imaging: Wavelength modulated spatially offset Raman spectroscopy with optical coherence tomography
复制标题
DOI：
10.1002/jbio.201700129
复制
发表时间：
2018-01-01
影响因子：
2.8
通讯作者：
Dholakia, Kishan
中科院分区：
文献类型：
--
作者：
Chen, Mingzhou;Mas, Josep;Dholakia, Kishan
生物光子学的一个主要挑战是多模态成像，以获得深度的形态和分子信息。我们展示了一种混合的方法集成光学相干断层扫描（OCT）与波长调制空间偏移拉曼光谱（WM-SORS）。与深度共定位从OCT获得的，我们可以穿透1.2毫米深到强散射介质（猪油），以获得高达14倍的增强的拉曼信号从一个隐藏的目标（聚苯乙烯）与空间偏移。我们的方法是能够检测的拉曼和OCT信号的药物颗粒嵌入混浊介质，并揭示了白色物质在0.6毫米厚的脑组织层内的深度。这种深度分辨的无标记多模式方法是分析复杂生物医学样品的有力途径。
A major challenge in biophotonics is multimodal imaging to obtain both morphological and molecular information at depth. We demonstrate a hybrid approach integrating optical coherence tomography (OCT) with wavelength modulated spatially offset Raman spectroscopy (WM-SORS). With depth colocalization obtained from the OCT, we can penetrate 1.2-mm deep into strong scattering media (lard) to acquire up to a 14-fold enhancement of a Raman signal from a hidden target (polystyrene) with a spatial offset. Our approach is capable of detecting both Raman and OCT signals for pharmaceutical particles embedded in turbid media and revealing the white matter at depth within a 0.6-mm thick brain tissue layer. This depth resolved label-free multimodal approach is a powerful route to analyze complex biomedical samples.