Efficient TALEN‐mediated gene editing in wheat
Efficient TALEN‐mediated gene editing in wheat
复制标题
TALEN 介导的小麦高效基因编辑
DOI：
--
复制
发表时间：
2019
影响因子：
13.8
通讯作者：
M. Ayliffe
中科院分区：
文献类型：
--
作者：
M. Luo;Hongyu Li;Soma Chakraborty;Robert Morbitzer;A. Rinaldo;N. Upadhyaya;Dhara Bhatt;Smitha Louis;Terese M Richardson;T. Lahaye;M. Ayliffe
植物基因组编辑是生产新的植物基因型别的重大进展。然而，之前只有一份报告将这项技术应用于使用TALEN介导的基因编辑来产生可遗传修饰的六倍体小麦(Wang等人，2014)。在这里，我们描述了面包小麦中一个uidA转基因基因和第二个内源基因lr21Ψ的高效TALEN编辑，其效率超过了以前在该物种中的TALEN和CRISPR/CAS9报告。这篇文章受版权保护。版权所有。
Plant genome editing is a major advance in the production of novel plant genotypes. There is, however, only a single previous report of applying this technology to hexaploid wheat using TALEN-mediated gene editing to produce heritable modifications (Wang et al., 2014). Here we describe highly efficient TALEN editing of a uidA transgene and a second endogenous gene, lr21Ψ, in bread wheat with efficiencies exceeding previous TALEN and CRISPR/Cas9 reports in this species. This article is protected by copyright. All rights reserved.