4D tracking of clinical seminal samples for quantitative characterization of motility parameters
4D tracking of clinical seminal samples for quantitative characterization of motility parameters
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DOI：
10.1364/boe.5.000690
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发表时间：
2014-03-01
影响因子：
3.4
通讯作者：
Dubois, Frank
中科院分区：
文献类型：
--
作者：
Di Caprio, Giuseppe;El Mallahi, Ahmed;Dubois, Frank
在这篇文章中，我们研究了使用部分空间相干照明的数字全息显微镜来自动检测和跟踪精子。这种体外分析定量参数的技术对评估精液质量是有用的。事实上，由于数字全息术的能力，所开发的算法允许我们解析被研究细胞的聚焦幅度和相位图，而不依赖于样本图像的焦平面。我们已经在临床精液样本上表征了细胞的运动性。特别是，对异常精子细胞进行了表征，并将其定量运动参数与正常精子进行了比较。(C)2014年美国光学学会
In this paper we investigate the use of a digital holographic microscope, with partial spatial coherent illumination, for the automated detection and tracking of spermatozoa. This in vitro technique for the analysis of quantitative parameters is useful for assessment of semen quality. In fact, thanks to the capabilities of digital holography, the developed algorithm allows us to resolve in-focus amplitude and phase maps of the cells under study, independently of focal plane of the sample image. We have characterized cell motility on clinical samples of seminal fluid. In particular, anomalous sperm cells were characterized and the quantitative motility parameters were compared to those of normal sperm. (C) 2014 Optical Society of America