Amplification of aptamer sensor signals by four orders of magnitude via interdigitated organic electrochemical transistors
Amplification of aptamer sensor signals by four orders of magnitude via interdigitated organic electrochemical transistors
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DOI：
10.1016/j.bios.2019.111668
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发表时间：
2019-11-01
影响因子：
12.6
通讯作者：
Mayer, Dirk
中科院分区：
文献类型：
--
作者：
Liang, Yuanying;Wu, Changtong;Mayer, Dirk
电化学适体受体/传感器系统是用于检测各种目标的新兴E-AB传感器（适体传感器）的关键要素。然而，这些电流传感器的性能通常受到传感器表面附着的受体密度低和背景信号高的限制。在目前的工作中，叉指有机电化学晶体管（iOECT）被用作传感器来提高适体传感器的灵敏度和动态检测范围。因此，电流传感器的电极被用作栅电极来操作 iOECT。该装置用于检测低重量目标分子三磷酸腺苷（ATP），这是一种常见的生物标志物，在心血管、神经退行性疾病和免疫缺陷疾病中发挥着重要作用。这种新型适体传感器可以以超高灵敏度选择性地检测浓度低至10 pM的ATP，这比使用电流传感器原理的相同适体传感器（检测限为106 nM）和大多数其他先前报道的电化学传感器的检测限低四个数量级。此外，还证明了传感器再生，这有助于 OECT 适体传感器的重复使用。小器件尺寸与高跨导相结合，为开发用于护理点应用的高灵敏度集成微型生物传感器铺平了道路。
Electrochemical aptamer receptor/transducer systems are key elements of emerging E-AB sensors (aptasensor) used for the detection of various kinds of targets. However, the performance of these amperometric sensors is often limited by the low density of receptors attached to the sensor surface and high background signals. In the present work, interdigitated organic electrochemical transistors (iOECT) were used as a transducer to enhance the sensitivity and dynamic detection range of aptasensors. Therefore, the electrode of an amperometric sensor was utilized as gate electrode to operate the iOECT. This device was used to detect the low weight target molecule adenosine triphosphate (ATP), a common biomarker, which plays an important role for cardiovascular, neurodegenerative, and immune deficiency diseases. The novel aptasensor can selectively detect ATP with ultrahigh sensitivity down to the concentration of 10 pM, which is four orders of magnitude lower than the detection limit of the same aptasensor using an amperometric transducer principle (limit-of-detection of 106 nM) and most other previously reported electrochemical sensors. Furthermore, sensor regeneration was demonstrated, which facilitates reusability of OECT aptasensors. The small device size in combination with high transconductances paves the way for the development of highly sensitive integrated micro-biosensors for point-of-care applications.