FVB/N mice are highly resistant to primary infection with Nippostrongylus brasiliensis.
FVB/N mice are highly resistant to primary infection with Nippostrongylus brasiliensis.
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DOI：
10.1017/s0031182008005192
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发表时间：
2009-01
期刊：
影响因子：
2.4
通讯作者：
Dent LA
中科院分区：
文献类型：
--
作者：
Knott ML;Hogan SP;Wang H;Matthaei KI;Dent LA
在小鼠原发性和继发性感染的肺前阶段，巴西尼波圆线虫幼虫特别容易受到免疫攻击。虽然大多数常见的实验室小鼠品系是寄生虫的允许宿主，但在本研究中，我们首次报道了naïve FVB/N小鼠对巴西乳螨的强抗性。在感染后的24小时内，对幼虫的损害是明显的，这可能对后来的幼虫发育和繁殖成功至关重要。在CBA/Ca易感小鼠和FVB/N耐药小鼠中，皮肤的炎症反应和幼虫从该组织中逃脱的情况相似，大多数幼虫在原发性感染后4小时内离开。不同菌株间的肺幼虫负荷相似，但从FVB/N小鼠中恢复的幼虫体积更小，运动能力更弱。在FVB/N小鼠中，肠道的幼虫定植受损，蠕虫产卵很少。然而，FVB/N小鼠对一种主要局限于肠道的线虫bakeri Heligmosomoides（也称为polygyrus Heligmosomoides）没有表现出增强的抗性。在巴西奈瑟菌感染的肺前或肺阶段造成的损伤可能会导致持续的发育和功能异常，这在感染的肠道阶段非常明显。
Nippostrongylus brasiliensis larvae are particularly susceptible to immunological attack during the pre-lung stage of primary and secondary infections in mice. Whilst most of the common laboratory strains of mice are permissive hosts for the parasite, in this study we report for the first time, the strong resistance of naïve FVB/N mice to N. brasiliensis. Damage to larvae is evident within the first 24 h of infection and this may be critical to later larval development and reproductive success. Inflammatory responses in the skin, and larval escape from this tissue were comparable in susceptible CBA/Ca and resistant FVB/N mice, with most larvae exiting within 4 h of a primary infection. Lung larval burdens were also similar between strains, but larvae recovered from FVB/N mice were smaller and less motile. In FVB/N mice, larval colonization of the gut was impaired and worms produced very few eggs. However FVB/N mice did not show enhanced resistance to Heligmosomoides bakeri (also known as Heligmosomoides polygyrus), a nematode largely restricted to the gut. Damage done in the pre-lung or lung stages of infection with N. brasiliensis is likely to contribute to ongoing developmental and functional abnormalities, which are profoundly evident in the gut phase of infection.