Effects of follicle-stimulating hormone and ovarian steroids during in vitro meiotic maturation on fertilization of rat oocytes.
Effects of follicle-stimulating hormone and ovarian steroids during in vitro meiotic maturation on fertilization of rat oocytes.
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DOI：
10.1002/mrd.1120230304
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发表时间：
1989-07
期刊：
影响因子：
--
通讯作者：
X. Zhang;D. Armstrong
中科院分区：
文献类型：
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作者：
X. Zhang;D. Armstrong
本研究考察了促性腺激素和卵巢类固醇在大鼠卵母细胞体外减数分裂成熟过程中对其体外受精能力的影响。从未成熟大鼠的窦卵泡中分离出成熟的卵母细胞，并在自然完成减数分裂成熟的条件下作为卵丘细胞复合物（OCC）进行培养。然后将它们暴露在精子中，使卵母细胞在体内成熟，表现出高受精率。与妊娠母马血清促性腺激素（PMSG）或促卵泡激素（FSH）处理大鼠的卵母细胞相比，未处理大鼠的卵母细胞发生生发囊泡破裂的比例相似（大于80%），但此类卵母细胞受精率较低（70% vs. 20%）。在体外成熟过程中，FSH的存在恢复了未经处理的大鼠卵母细胞的受精率，而细胞色素P450抑制剂氨基酰硫胺磷酸盐则消除了FSH的这种有益作用。在体外成熟期添加黄体酮可重复FSH对未处理大鼠卵母细胞受精率的有益影响；雌二醇-17 β在这方面效果较差，而5 α -二氢睾酮则无效。这些结果表明，卵泡刺激素和黄体酮虽然对卵母细胞的核成熟没有明显的影响，但在卵母细胞成熟过程中对正常受精的发生起着重要的作用。
The present study examined the effects of gonadotropins and ovarian steroids during in vitro meiotic maturation of rat oocytes on their ability to undergo in vitro fertilization. Fully grown oocytes were isolated from antral follicles of immature rats and cultured as oocyte-cumulus cell complexes (OCC) under conditions in which completion of meiotic maturation occurs spontaneously. They were then exposed to spermatozoa under conditions in which oocytes matured in vivo exhibit high fertilization rates. Compared with oocytes from pregnant mare's serum gonadotropin (PMSG) or follicle-stimulating hormone (FSH)-treated rats, a similar proportion of the oocytes (greater than 80%) from untreated rats underwent germinal vesicle breakdown, but such oocytes had a lower rate of fertilization (70% vs. 20%). The presence of FSH during in vitro maturation restored the fertilization rate for oocytes from untreated rats, while a cytochrome P450 inhibitor, aminoglutethimide phosphate abolished this beneficial effect of FSH. The addition of progesterone during the in vitro maturation period duplicated the beneficial effect of FSH on fertilization rate of oocytes from untreated rats; oestradiol-17 beta was less effective in this regard, and 5 alpha-dihydrotestosterone was ineffective. These findings indicate that FSH and progesterone, although having no apparent effect on nuclear maturation of the oocyte, play an important role during oocyte maturation in enabling normal fertilization to occur.