Cloning and expression analysis of a putative ABC transporter gene BgABC1 from the biotrophic pathogenic fungus Blumeria graminis f. sp tritici
Cloning and expression analysis of a putative ABC transporter gene BgABC1 from the biotrophic pathogenic fungus Blumeria graminis f. sp tritici
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DOI：
10.1111/j.1439-0434.2007.01332.x
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发表时间：
2008-02-01
影响因子：
1.5
通讯作者：
Ma, Z.
中科院分区：
文献类型：
--
作者：
Hu, W.;Yan, L.;Ma, Z.
从小麦白粉病菌Blumeria graminis f.小麦种（Bgt）. BgABC 1的氨基酸序列分析表明，BgABC 1蛋白在N端有一个保守的核苷酸结合折叠，在C端有6个跨膜结构域（TMD）。使用实时聚合酶链反应的BgABC 1表达的分析表明，该基因的表达显着增加时，真菌生长在小麦幼苗与14 α-去甲基酶抑制剂，三唑酮处理。结果表明，BgABC 1基因参与了该菌对杀菌剂毒性的保护作用。
A putative ATP-binding cassette (ABC) transporter gene (BgABC1) was isolated from the biotrophic pathogenic fungus Blumeria graminis f. sp. tritici (Bgt). Analysis of the deduced amino acid sequence of BgABC1 showed that the BgABC1 protein had a conserved nucleotide-binding fold in the N-terminus, and six transmembrane domains (TMDs) in the C-terminus. Analysis of the BgABC1 expression using real-time polymerase chain reaction showed that expression of the gene was increased significantly when the fungus was growing in wheat seedlings treated with 14 alpha-demethylase-inhibiting fungicide, triadimefon. The results indicated that the BgABC1 was involved in the protection of the fungus against fungicide toxicity.