Identification of the sex-determining locus in the Thai medaka, Oryzias minutillus
Identification of the sex-determining locus in the Thai medaka, Oryzias minutillus
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DOI：
10.1159/000125839
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发表时间：
2008-01-01
影响因子：
1.7
通讯作者：
Sakaizumi, M.
中科院分区：
文献类型：
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作者：
Nagai, T.;Takehana, Y.;Sakaizumi, M.
在青鳉 Oryzias latipes 中发现了一种性别决定基因 DMY，它与哺乳动物中的 SRY 基因相当。尽管与 O. latipes 密切相关的 Oryzias curvinotus 物种也具有 DMY，但在其他 Oryzias 鱼类中尚未发现该基因。最近的研究表明，Oryzias dancena 和 Oryzias hubbsi 的性染色体与 O. latipes 不同，并且这些物种分别具有 XX/XY 和 ZZ/ZW 系统。这可能表明 Oryzias 物种在不同的性染色体上进化出了不同的性别决定基因。在本研究中，我们研究了Oryzias minutillus 的性别决定机制，它与O. dancena 和O. hubbsi 密切相关。使用 14 个分离的性别连锁 DNA 标记进行连锁分析表明，该物种具有 XX/XY 性别决定系统。这些性连锁标记位于 O. latipes 的连锁群 8 上，表明 O. minutillus 的性染色体与其他 Oryzias 物种的常染色体同源。此外，使用紧密性别连锁标记的荧光原位杂交表明，O. minutillus 和 O. dancena 的 XY 性染色体不是同源的。这些发现为这些密切相关的物种中性染色体和性别决定基因的独立起源提供了额外的证据。版权所有 (C) 2008 S. Karger AG，巴塞尔。
A sex-determining gene, DMY, which is comparable to the SRY gene in mammals, has been identified in the medaka, Oryzias latipes. Although Oryzias curvinotus, a closely related species to O. latipes also has DMY, this gene has not been found in other Oryzias fishes. It has recently been demonstrated that the sex chromosomes of Oryzias dancena and Oryzias hubbsi differ from those of O. latipes and these species have XX/XY and ZZ/ZW systems, respectively. This may suggest that Oryzias species have evolved different sex-determining genes on different sex chromosomes. In the present study, we investigated the sex determination mechanism in Oryzias minutillus, which is closely related to O. dancena and O. hubbsi. Linkage analysis using 14 isolated sex-linked DNA markers showed that this species has an XX/XY sex determination system. These sex-linked markers were located on linkage group 8 of O. latipes, suggesting that the sex chromosomes of O. minutillus are homologous to the autosomes of other Oryzias species. Furthermore, fluorescence in situ hybridization using a tightly sex-linked marker demonstrated that the XY sex chromosomes of O. minutillus and O. dancena were not homologous. These findings provide additional evidence for independent origins of sex chromosomes and sex-determining genes in these closely related species. Copyright (C) 2008 S. Karger AG, Basel.