Surface plasmon resonance imaging system with Mach-Zehnder phase-shift interferometry for DNA micro-array hybridization
Surface plasmon resonance imaging system with Mach-Zehnder phase-shift interferometry for DNA micro-array hybridization
复制标题
用于 DNA 微阵列杂交的马赫曾德尔相移干涉表面等离子体共振成像系统
DOI：
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
C.
中科院分区：
文献类型：
--
作者：
Feng;Shean;Chien;Chia;Y. Su;G. Lin;K.;Jin;W. Ku;S. Chiu;C.
表面等离子体共振（SPR）成像系统是一种基于改进的马赫-曾德尔相移干涉术（PSI）的生物分子相互作用分析（BIA）新技术，它可以测量生物分子相互作用中共振反射光的空间相位变化。该技术可同时检测上千个探针NDA点的微阵列SPR生物传感器。微阵列SPR生物传感器由于其快速、可行的特性，在蛋白质非特异性吸附、膜/蛋白质相互作用、DNA杂交等方面有着广泛的应用前景。与传统SPR成像系统相比，SPR PSI成像系统的检测灵敏度提高到每个光斑约1 pg/mm 2。SPR PSI成像系统及其SPR传感器已成功地用于观察轻微的指数变化的后果，氩气流经氮气在真实的时间，具有高灵敏度，并在高通过筛选率。
Surface plasmon resonance (SPR) imaging system is presented as a novel technique based on modified Mach-Zehnder phase-shifting interferometry (PSI) for biomolecular interaction analysis (BIA), which measures the spatial phase variation of a resonantly reflected light in biomolecular interaction. In this technique, the micro-array SPR biosensors with over a thousand probe NDA spots can be detected simultaneously. Owing to the feasible and swift measurements, the micro-array SPR biosensors can be extensively applied to the nonspecific adsorption of protein, the membrane/protein interactions, and DNA hybridization. The detection sensitivity of the SPR PSI imaging system is improved to about 1 pg/mm2 for each spot over the conventional SPR imaging systems. The SPR PSI imaging system and its SPR sensors have been successfully used to observe slightly index change in consequence of argon gas flow through the nitrogen in real time, with high sensitivity, and at high-throughout screening rates.
影响因子：
7.4
作者：
Nelson, BP;Grimsrud, TE;Corn, RM
通讯作者：
Corn, RM