The Burst Size Distribution in the Growth of Bacterial Viruses (Bacteriophages)
The Burst Size Distribution in the Growth of Bacterial Viruses (Bacteriophages)
复制标题
细菌病毒（噬菌体）生长中的爆发大小分布
作者：
M. Delbrück
伯内特（1929）发明了一种测量单个细菌病毒产量（爆发量）的方法。它包括稀释感染细菌的悬浮液，并从稀释的悬浮液中培养大量样品。每个样本平均应含有少于一个受感染的细菌。只有一小部分样本会含有一种以上的感染细菌。孵育样品直至所有细菌裂解，然后铺板。伯内特没有在足够大的规模上应用这种技术，以表明单个爆破尺寸的分布。Ellis和Delbriick（1939）以及Delbriick（1942）得到的结果表明，爆发大小的变化非常大。然而，这一系列的实验既不够大，也不够准确，无法给出爆发大小分布的清晰概念。在技术上作了一些小的改进后，我们现在已经成功地得到了分布的一个合理的近似。
Burnet (1929) invented a method for measuring the yield of virus from individual bacteria (burst size). It consists in diluting a suspension of infected2 bacteria and incubating a large number of samples from the diluted suspension. Each sample should contain on the average less than one infected bacterium. Only a small fraction of the samples will then contain more than one infected bacterium. The samples are incubated until all bacteria are lysed, and then plated. Burnet did not apply this technique on a scale sufficiently large to indicate the distribution of individual burst sizes. Ellis and Delbriick (1939) and Delbriick (1942) obtained results which indicated an exceedingly wide variation of the burst sizes. However, these series of experiments were neither sufficiently large nor sufficiently accurate to give a clear idea of the burst size distribution. With some minor improvements in technique we have now succeeded in obtaining a fair approximation of the distribution.