Treatment of Akata EBV-Positive Cells with Doxorubicin Causes More EBV Reactivation than Treatment with Etoposide
Treatment of Akata EBV-Positive Cells with Doxorubicin Causes More EBV Reactivation than Treatment with Etoposide
复制标题
DOI：
10.1159/000323627
复制
发表时间：
2011-01-01
期刊：
影响因子：
3.3
通讯作者：
Helena Vasconcelos, M.
中科院分区：
文献类型：
--
作者：
Lima, Raquel T.;Seca, Hugo;Helena Vasconcelos, M.
背景/目的：EB病毒与伯基特淋巴瘤（BL）有关。它建立了一个潜伏感染，但它的重新激活已被观察到在接受长期化疗的患者。阿霉素对病毒再活化的作用以前已有描述，但依托泊苷或阿糖胞苷对EBV再活化的作用尚未在文献中报道。这项工作的目的就是进行这样的研究。方法：用足叶乙甙、阿霉素或阿糖胞苷处理Akata EBV阳性细胞系。通过台盼蓝分析活细胞，通过TUNEL分析程序性细胞死亡，通过RT-PCR分析mRNA水平，通过Western印迹分析细胞或病毒蛋白。通过电子显微镜观察病毒。结果：所有研究的药物均通过凋亡引起细胞死亡。比较依托泊苷和多柔比星（在其IC 50）在EBV阳性细胞中的作用，依托泊苷引起的EBV再活化比多柔比星少。阿糖胞苷显然没有重新激活EBV。结论：当用下列药物的各自的IC 50处理Akata EBV阳性细胞时，依托泊苷诱导的EBV再活化比多柔比星少，阿糖胞苷显然不诱导EBV再活化。版权所有（C）2011 S. Karger AG，巴塞尔
Background/Aims: EBV has been associated with Burkitt lymphoma (BL). It establishes a latent infection but its reactivation has been observed in patients receiving long-term chemotherapy. The effect of doxorubicin on virus reactivation has been described previously, but the effect of etoposide or cytarabine on EBV reactivation has not been reported in the literature. The aim of this work was to carry out such a study. Methods: Akata EBV-positive cell lines were treated with etoposide, doxorubicin or cytarabine. Viable cells were analyzed by trypan blue, programmed cell death by TUNEL assay, mRNA levels by RT-PCR and cellular or viral proteins by Western blot. Viruses were visualized by electron microscopy. Results: All of the studied drugs caused cell death by apoptosis. Comparing the effect of etoposide and doxorubicin (at their IC 50) in the EBV-positive cells, etoposide caused less EBV reactivation than doxorubicin. Cytarabine apparently did not reactivate EBV. Conclusion: When treating Akata EBV-positive cells with the respective IC 50 of the following drugs, etoposide induced less EBV reactivation than doxorubicin, and cytarabine apparently did not induce EBV reactivation. Copyright (C) 2011 S. Karger AG, Basel