Molecular cloning, complete sequence of the small subunit ribosomal RNA coding region and phylogeny of Toxoplasma gondii.
Molecular cloning, complete sequence of the small subunit ribosomal RNA coding region and phylogeny of Toxoplasma gondii.
复制标题
弓形虫小亚基核糖体 RNA 编码区的分子克隆、完整序列和系统发育。
DOI：
10.1016/0166-6851(93)90037-x
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发表时间：
1993
影响因子：
1.5
通讯作者：
Gajadhar,AA
中科院分区：
文献类型：
--
作者：
Gagnon,S;Levesque,RC;Sogin,ML;Gajadhar,AA
从弓形虫RH株中分离到完整的16s样rRNA基因。t的编码区。从两个不同实验室获得的gondii16s样rDNA长度为1796个核苷酸。与先前确定的t比较。Gondiipartial序列在57个位置上存在核苷酸差异。系统发育分析将球虫中的5种原生动物归为单系群。murisist的单一性。gondii被100%的bootstrapping值所证实。
The complete 16S-like rRNA gene was isolated from the RH strain ofToxoplasma gondiiand compared with that of other organisms. The coding region of theT. gondii16S-like rDNA obtained from 2 different laboratories was 1796 nucleotides long. Comparison with previously determinedT. gondiipartial sequences showed nucleotide differences at 57 positions. Phylogenetic analysis placed 5 protozoa from the coccidia into a monophyletic group. The monophyly ofSarcocystis murisandT. gondiiwas confirmed by bootstrapping values of 100%.