Isolation of Hox Cluster Genes from Insects Reveals an Accelerated Sequence Evolution Rate
Isolation of Hox Cluster Genes from Insects Reveals an Accelerated Sequence Evolution Rate
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DOI：
10.1371/journal.pone.0034682
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发表时间：
2012-06-07
期刊：
影响因子：
3.7
通讯作者：
Schierwater, Bernd
中科院分区：
文献类型：
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作者：
Hadrys, Heike;Simon, Sabrina;Schierwater, Bernd
在基因家族中，是HOX基因，在后生动物中，昆虫(六足类)在研究发育进化方面引起了特别的关注。然而，令人惊讶的是，在35个昆虫目中的26个目中还没有分离到HOX基因，现有的序列主要来自两个目(61%来自膜翅目，22%来自双翅目)。我们设计了昆虫特异的引物，从6个昆虫目中分离到37个新的HOX簇基因的部分同源盒序列(Lab、PB、Hox3、FTZ、ANTP、Scr、Abd-a、Abd-B、Dfd和Ubx)。这些新的基因序列为昆虫中HOX基因的比较研究提供了第一步。此外，同源框序列的比较距离分析揭示了基因分歧率和物种辐射成功率之间的相关性，昆虫显示出同源框序列进化的最高速率。
Among gene families it is the Hox genes and among metazoan animals it is the insects (Hexapoda) that have attracted particular attention for studying the evolution of development. Surprisingly though, no Hox genes have been isolated from 26 out of 35 insect orders yet, and the existing sequences derive mainly from only two orders (61% from Hymenoptera and 22% from Diptera). We have designed insect specific primers and isolated 37 new partial homeobox sequences of Hox cluster genes (lab, pb, Hox3, ftz, Antp, Scr, abd-a, Abd-B, Dfd, and Ubx) from six insect orders, which are crucial to insect phylogenetics. These new gene sequences provide a first step towards comparative Hox gene studies in insects. Furthermore, comparative distance analyses of homeobox sequences reveal a correlation between gene divergence rate and species radiation success with insects showing the highest rate of homeobox sequence evolution.