Characterization of a Wheat Breeders' Array suitable for high-throughput SNP genotyping of global accessions of hexaploid bread wheat (Triticum aestivum).
Characterization of a Wheat Breeders' Array suitable for high-throughput SNP genotyping of global accessions of hexaploid bread wheat (Triticum aestivum).
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DOI：
10.1111/pbi.12635
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发表时间：
2017-03
影响因子：
13.8
通讯作者：
Edwards KJ
中科院分区：
文献类型：
--
作者：
Allen AM;Winfield MO;Burridge AJ;Downie RC;Benbow HR;Barker GL;Wilkinson PA;Coghill J;Waterfall C;Davassi A;Scopes G;Pirani A;Webster T;Brew F;Bloor C;Griffiths S;Bentley AR;Alda M;Jack P;Phillips AL;Edwards KJ
定向选择和近交导致了六倍体面包小麦优良种质遗传多样性的缺乏。多样性减少可能是培育高产品种的一个限制因素，最重要的是，它可能意味着面对不断变化的气候和资源压力的复原力降低。最近的技术进步使分子标记的发展成为可能，用于评估和利用六倍体小麦的遗传多样性。从收集了819-571个已有特征的小麦标记入手，本文介绍了35-143个单核苷酸多态标记，它们非常适合于六倍体小麦优良材料的基因分型。为了评估它们的适合性，这些标记已经使用商业高密度Affymetrix Axiom®基因分型阵列(小麦育种者阵列)在高通量384微孔板配置中进行了验证，以鉴定包括地方品种和来自商业育种社区的优质品系在内的全球小麦种质的多样性。我们证明，小麦育种者阵列也适用于生成以前未表征的群体的高密度遗传图谱，并表征由突变产生的新的遗传多样性。为了方便小麦群体使用该阵列，已通过互动网站‘CerealsDB’提供了标记、相关序列和基因信息。
Targeted selection and inbreeding have resulted in a lack of genetic diversity in elite hexaploid bread wheat accessions. Reduced diversity can be a limiting factor in the breeding of high yielding varieties and crucially can mean reduced resilience in the face of changing climate and resource pressures. Recent technological advances have enabled the development of molecular markers for use in the assessment and utilization of genetic diversity in hexaploid wheat. Starting with a large collection of 819 571 previously characterized wheat markers, here we describe the identification of 35 143 single nucleotide polymorphism‐based markers, which are highly suited to the genotyping of elite hexaploid wheat accessions. To assess their suitability, the markers have been validated using a commercial high‐density Affymetrix Axiom® genotyping array (the Wheat Breeders’ Array), in a high‐throughput 384 microplate configuration, to characterize a diverse global collection of wheat accessions including landraces and elite lines derived from commercial breeding communities. We demonstrate that the Wheat Breeders’ Array is also suitable for generating high‐density genetic maps of previously uncharacterized populations and for characterizing novel genetic diversity produced by mutagenesis. To facilitate the use of the array by the wheat community, the markers, the associated sequence and the genotype information have been made available through the interactive web site ‘CerealsDB’.