Differential expression of placental and vascular endothelial nitric oxide synthase in an ovine model of fetal growth restriction
Differential expression of placental and vascular endothelial nitric oxide synthase in an ovine model of fetal growth restriction
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DOI：
10.1016/j.ajog.2006.06.018
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发表时间：
2006-09-01
影响因子：
9.8
通讯作者：
Galan, Henry L.
中科院分区：
文献类型：
--
作者：
Arroyo, Juan A.;Anthony, Russell V.;Galan, Henry L.
目的：这项研究的目的是评估内皮型一氧化氮合酶的浓度在胎盘，子宫，和脐带血管近期在一个绵羊模型的子宫内生长受限的高温诱导开始在早期pregnancy.Study设计：四个怀孕的母羊暴露于高温条件下80天开始在妊娠35天，以诱导子宫内生长受限。将四只母羊保持在环境条件下作为对照。计算脐动脉多普勒收缩舒张比。在妊娠128天，放置胎儿导管进行主动脉血压测量和血气测定。在妊娠132天，测定胎儿平均全身血压和气体。结果：与对照组相比，宫内生长受限组胎儿和胎盘重量明显减轻（P
Objective: The objective of the study was to evaluate endothelial nitric oxide synthase concentration in the placenta, uterine, and umbilical vessels near term in an ovine model of intrauterine growth restriction induced by hyperthermia beginning in early gestation.Study design: Four pregnant ewes were exposed to hyperthermia conditions for 80 days beginning at 35 days gestation to induce intrauterine growth restriction. Four ewes were kept in ambient conditions as controls. Umbilical artery Doppler systolic to diastolic ratios were calculated. At 128 days gestation, fetal catheters were placed for aortic blood pressure measurements and blood gas determination. At 132 days gestation, fetal mean systemic blood pressure and gases were determined. Sheep placentomes, umbilical artery and vein, and uterine artery were assessed for endothelial nitric oxide synthase concentration and immunolocalization.Results: Compared with control pregnancies, the intrauterine growth restriction pregnancies showed: (1) reduced fetal and placental weights (P