Quantitative and qualitative preparations of bacterial outer membrane vesicles.
Quantitative and qualitative preparations of bacterial outer membrane vesicles.
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DOI：
10.1007/978-1-62703-245-2_16
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Kuehn, Meta J
中科院分区：
文献类型：
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作者：
Chutkan, Halima;Macdonald, Ian;Manning, Andrew;Kuehn, Meta J
革兰氏阴性细菌外膜囊泡的生产和功能已经使用各种定量和定性方法进行了研究。使用不纯的囊泡制剂会妨碍这些类型的分析。在这里，我们描述了一套技术，是有用的定量分析的囊泡生产和制备产量的高度纯化的囊泡的囊泡功能或组成的研究。程序和建议也包括从封装和低产量菌株的囊泡纯化。
Gram-negative bacterial outer membrane vesicle production and function have been studied using a variety of quantitative and qualitative methods. These types of analyses can be hampered by the use of impure vesicle preparations. Here we describe a set of techniques that are useful for the quantitative analysis of vesicle production and for preparative yields of highly purified vesicles for studies of vesicle function or composition. Procedures and advice are also included for the purification of vesicles from encapsulated and low-yield strains.