DECREASED EXPRESSION OF DCC MESSENGER-RNA IN HUMAN COLORECTAL CANCERS
DECREASED EXPRESSION OF DCC MESSENGER-RNA IN HUMAN COLORECTAL CANCERS
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DOI：
10.1002/ijc.2910530215
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发表时间：
1993-01-21
影响因子：
6.4
通讯作者：
YACHI, A
中科院分区：
文献类型：
--
作者：
ITOH, F;HINODA, Y;YACHI, A
采用RT-PCR方法检测大肠癌患者手术切除组织中DCC基因mRNA的表达水平。DCC基因是从大肠癌染色体18 q缺失区克隆的抑癌基因。选择该方法是因为DCC mRNA的表达水平低于Northern印迹分析的可检测水平。DCC mRNA的半定量测量基于通过DCC cDNA的连续稀释定义的标准曲线进行。结果发现，DCC mRNA的表达水平在30例大肠癌中有17例低于癌旁组织。观察到组织标本中包含平滑肌对癌性和非癌性区域之间的比较几乎没有干扰作用。此外，4例大肠癌肝转移标本中DCC mRNA表达水平均降低，提示癌组织中DCC功能缺失可能在转移过程中起重要作用。
The mRNA expression levels of DCC gene, which is cloned from the deleted region of chromosome 18q in colorectal cancers and thought to be a tumor-suppressor gene, was evaluated in tissue specimens surgically resected from patients with colorectal cancer by RT-PCR. This method was chosen as the expression level of DCC mRNA is below the detectable level for Northern-blot analysis. Semi-quantitative measurements of DCC mRNA were performed based on a standard curve defined by serial dilution of DCC cDNA. As a result, the expression level of DCC mRNA was found to be lower in 17 out of 30 colorectal cancers than in adjacent non-cancerous tissues. Inclusion of smooth muscle in tissue specimens was observed to have little disturbing effect on comparisons between cancerous and noncancerous regions. In addition, all 4 specimens of colorectal cancer with liver metastasis showed the decreased expression level of DCC mRNA, suggesting that functional loss of DCC in cancerous tissues may play an important role in metastatic events.