Aluminium-inhibited NO3- uptake is related to Al-increased H2O2 content and Al-decreased plasma membrane ATPase activity in the root tips of Al-sensitive black soybean.
Aluminium-inhibited NO3- uptake is related to Al-increased H2O2 content and Al-decreased plasma membrane ATPase activity in the root tips of Al-sensitive black soybean.
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DOI：
10.1071/fp15289
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发表时间：
2017-02
期刊：
影响因子：
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通讯作者：
Dan Yang;Dongjie Chen;Ping Wang;Dai-hua Jiang;Huini Xu;Xiaolu Pang;Limei Chen;Yongxiong Yu;Kunzhi Li
中科院分区：
文献类型：
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作者：
Dan Yang;Dongjie Chen;Ping Wang;Dai-hua Jiang;Huini Xu;Xiaolu Pang;Limei Chen;Yongxiong Yu;Kunzhi Li
本研究以铝敏感黑豆(Glyine max(L.)(Merr.)标本在含有50-400微米铝的Hoagland溶液中处理1-4天。硝酸根吸收的测定表明，随着铝浓度的增加和处理时间的延长，大豆对硝酸根的吸收逐渐减少，说明铝胁迫显著降低了大豆对硝酸根的吸收。100µM铝胁迫4d后，大豆根尖质膜ATPase活性(无机磷释放)、H+泵活性、质膜ATPase磷酸化及其与14-3-3蛋白的相互作用均低于对照植株。在铝处理溶液中添加150µM镁离子显著缓解了铝对大豆吸收硝酸根的抑制作用。在100微米铝溶液中加入镁离子显著提高了大豆根尖质膜ATPase活性、H+泵活性、质膜ATPase的磷酸化及其与14-3-3蛋白的相互作用。在铝处理溶液中添加2 mM抗坏血酸(抗坏血酸，一种过氧化氢清除剂)，显著降低了铝抑制大豆对硝酸根的吸收。2 mM ASA和100µM Al共处理显著降低了大豆根尖中H_2O_2的积累，提高了质膜ATPase活性、H~+泵活性、质膜H~+-ATPase的磷酸化及其与14-3-3蛋白的相互作用。这些证据表明，铝抑制大豆根尖N3-吸收与增加大豆根尖中H_2O_2含量和降低PM-ATPase的磷酸化及其与14-3-3蛋白的相互作用和PM-ATPase活性有关。
In this study, Al-sensitive black soybean (Glycine max (L.) Merr.) specimens were treated in Hoagland solutions containing 50-400µM Al for 1-4 days. The measurement for NO3- uptake showed that the NO3- uptake decreased gradually as the Al concentration and treatment time increased, suggesting that Al stress significantly reduced the NO3- uptake by soybean. Under 100-µM Al stress for 4 days, the plasma membrane (PM) ATPase activity (inorganic phosphate (Pi) release), H+ pump activity, phosphorylation of PM ATPase and its interaction with 14-3-3 protein in soybean root tips were all smaller than those in the root tips of control plants. The addition of 150µM Mg2+ in Al treatment solutions significantly alleviated the Al inhibition of NO3- uptake in soybean. The presence of Mg2+ in a 100-µM Al solution pronouncedly enhanced PM ATPase activity, H+ pump activity, phosphorylation of PM ATPase and its interaction with 14-3-3 protein in soybean root tips. The application of 2mM ascorbic acid (AsA, an H2O2 scavenger) in Al treatment solutions significantly decreased Al-inhibited NO3- uptake in soybean. The cotreatment of soybeans with 2mM AsA and 100µM Al significantly reduced H2O2 accumulation and increased the PM ATPase activity, H+ pump activity, phosphorylation of PM H+-ATPase and its interaction with 14-3-3 protein in soybean root tips. The evidence suggested that Al-inhibited NO3- uptake is related to Al-increased H2O2 content and Al-decreased phosphorylation of PM ATPase and its interaction with 14-3-3 protein as well as PM ATPase activity in the root tips of soybean.