Relation between UV suppression of polarity in phi X174 and UV sensitivity of rho mutants.
Relation between UV suppression of polarity in phi X174 and UV sensitivity of rho mutants.
复制标题
phi X174 中极性的紫外线抑制与 rho 突变体的紫外线敏感性之间的关系。
DOI：
10.1128/jvi.37.3.955-962.1981
复制
发表时间：
1981
影响因子：
5.4
通讯作者：
Tessman,I
中科院分区：
文献类型：
--
作者：
Fassler,JS;Tessman,I
The suppression of polarity by UV irradiation was similar to the suppression by rho mutants. This was demonstrated for a polar nonsense mutant of phage phi X174. Treatment of the host for 30 min with 100 micrograms of the radiomimetic drug mitomycin C per ml was about as effective as 550 J of UV irradiation per m2 in relieving polarity. The shape of the UV survival curves for rho mutants could be linked to a proposed mechanism of UV relief of polarity. Host cell reactivation of phage lambda and W-reactivation of phage G4 were unaffected by rho mutations. UV suppression of polarity is independent of the Hcr- and RecA- phenotypes. An explanation for the UV sensitivity of rho mutants is provided, and several ways are considered in which UV irradiation may deplete cellular rho activity and thereby cause UV relief of polarity. We propose a novel theory that relates the UV inactivation of normal repair-proficient cells to a decrease in rho activity.
