Inhibition of Eimeria tenella development in vitro mediated by chicken macrophages and fibroblasts treated with chicken cell supernatants with IFN-γ activity
Inhibition of Eimeria tenella development in vitro mediated by chicken macrophages and fibroblasts treated with chicken cell supernatants with IFN-γ activity
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DOI：
10.2307/1592473
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发表时间：
1998-04-01
期刊：
影响因子：
1.4
通讯作者：
Bout, DT
中科院分区：
文献类型：
--
作者：
Dimier, IH;Quéré, P;Bout, DT
鸡巨噬细胞或成纤维细胞预处理的上清液来自concanavin a刺激的脾脏细胞或来自病毒转化的细胞系网状内皮病毒作为干扰素γ (ifn - γ)的来源，减缓随后的孢子子复制在细胞中。为了确定ifn - γ的存在，我们结合了ifn - γ的四种典型活性：抑制水泡性口炎病毒对ifn - γ处理的成纤维细胞的细胞病变作用，ifn - γ活化的巨噬细胞的细胞抑制活性，诱导ifn - γ活化的成纤维细胞和巨噬细胞的主要组织相容性复合体II抗原表达，以及诱导巨噬细胞产生亚硝酸盐。我们已经证明，一旦鸡成纤维细胞和巨噬细胞能够阻止含有强ifn - γ活性的培养上清激活后的柔嫩艾美耳球虫的细胞内复制，它们就具有微生物抑制能力。
Pretreatment of chicken macrophages or fibroblasts with supernatants from concanavalin A-stimulated spleen cells or from the virus-transformed cell line reticuloendotheliovirus as source of interferon gamma (IFN-gamma) slows down subsequent sporozoite replication in the cells. To identify the presence of IFN-gamma, we combined four typical activities of IFN-gamma: inhibition of cytopathic effect of vesicular stomatitis virus on IFN-gamma-treated fibroblasts, cytostatic activity of IFN-gamma-activated macrophages, induction of major histocompatibility complex II antigen expression on IFN-gamma-activated fibroblasts and macrophages, and induction of nitrite production in macrophages. We have shown that chicken fibroblasts and macrophages possess a microbiostatic capacity once they are able to prevent the otherwise unchecked intracellular replication of Eimeria tenella following activation with culture supernatants identified as containing a strong IFN-gamma activity.