A protein complex containing Mei5 and Sae3 promotes the assembly of the meiosis-specific RecA homolog Dmc1
A protein complex containing Mei5 and Sae3 promotes the assembly of the meiosis-specific RecA homolog Dmc1
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DOI：
10.1016/j.cell.2004.10.031
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发表时间：
2004-12-29
期刊：
影响因子：
64.5
通讯作者：
Shinohara, A
中科院分区：
文献类型：
--
作者：
Hayase, A;Takagi, M;Shinohara, A
减数分裂重组需要减数分裂特异性 RecA 同源物 Dmc1 以及有丝分裂 RecA 同源物 Rad51。在这里，我们表明，在染色体上，包括 Dmc1 与重组热点的关联，两种减数分裂特异性蛋白 Mei5 和 Sae3 对于 Dmc1 的组装是必需的，但对于 Rad51 则不是必需的。 Mei5、Sae3 和 Dmc1 形成三元进化保守复合体，需要 Rad51 招募到染色体。 Mei5、Sae3 和 Dmc1 的染色体关联相互依赖，它们的缺失会阻止 Rad51 丝的分解。我们的结果表明，Mei5 和 Sae3 是 Dmc1 重组酶的负载因子，并且 Dmc1-Mei5-Sae3 复合物整合到 Rad51 整体上，并与 Rad51 一起在减数分裂过程中的同源重组中发挥催化和结构作用。
Meiotic recombination requires the meiosis-specific RecA homolog Dmc1 as well as the mitotic RecA homolog Rad51. Here, we show that the two meiosisspecific proteins Mei5 and Sae3 are necessary for the assembly of Dmc1, but not for Rad51, on chromosomes including the association of Dmc1 with a recombination hot spot. Mei5, Sae3, and Dmc1 form a ternary and evolutionary conserved complex that requires Rad51 for recruitment to chromosomes. Mei5, Sae3, and Dmc1 are mutually dependent for their chromosome association, and their absence prevents the disassembly of Rad51 filaments. Our results suggest that Mei5 and Sae3 are loading factors for the Dmc1 recombinase and that the Dmc1-Mei5-Sae3 complex is integrated onto Rad51 ensembles and, together with Rad51, plays both catalytic and structural roles in interhomolog recombination during meiosis.