Ultrastructural localization of calcium in psoriatic and normal human epidermis.
Ultrastructural localization of calcium in psoriatic and normal human epidermis.
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DOI：
10.1001/archderm.1991.01680010067010
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发表时间：
1991
影响因子：
--
通讯作者：
G. Menon;P. Elias
中科院分区：
文献类型：
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作者：
G. Menon;P. Elias
与离子捕获细胞化学，我们以前证明了钙离子在小鼠表皮的分布，这种离子在调节表皮分化的作用一致的模式。由于已知银屑病的增殖和分化缺陷，我们比较了受累与未受累银屑病皮损和正常人表皮的钙分布。而正常人和未参与的银屑病表皮显示增加的含钙沉淀物在最上层的颗粒层，相比之下，银屑病病变的基底层含有较少的细胞外钙，有利于增强增殖的条件。此外，所有银屑病基底上细胞层显示比正常浓度的钙更重，表明程序终末分化的正常钙梯度的损失。这种异常的轮廓可以解释银屑病中发生的分化缺陷（如角化不全）。最后，银屑病病变显示保留离子钙在上层颗粒细胞间的领域，没有正常的细胞间双层，发现可能是银屑病异常脱屑和渗透性屏障的基础。
With ion capture cytochemistry, we previously demonstrated the distribution of calcium ions in murine epidermis, a pattern consistent with a role for this ion in the regulation of epidermal differentiation. Because of the known proliferation and differentiation defects in psoriasis, we compared the calcium distribution of involved vs uninvolved psoriatic lesions and normal human epidermis. Whereas normal human and uninvolved psoriatic epidermis revealed increased calcium-containing precipitates in the uppermost stratum granulosum, in contrast the basal layer of psoriatic lesions contained less extracellular calcium, a condition that favored enhanced proliferation. Moreover, all psoriatic suprabasal cell layers displayed heavier than normal concentrations of calcium, indicating loss of the normal calcium gradient that programs terminal differentiation. This abnormal profile may account for the differentiation defects (eg, parakeratosis) that occur in psoriasis. Finally, psoriatic lesions displayed retained ionic Ca in intercellular domains of the upper stratum granulosum with absence of normal intercellular bilayers, findings that may underlie the abnormal desquamation and permeability barrier in psoriasis.