Sphere formation restores and confers hair-inducing capacity in cultured mesenchymal cells
Sphere formation restores and confers hair-inducing capacity in cultured mesenchymal cells
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DOI：
10.1111/j.1600-0625.2011.01281.x
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发表时间：
2011-08-01
影响因子：
3.6
通讯作者：
Kishi, Kazuo
中科院分区：
文献类型：
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作者：
Shimizu, Ruka;Okabe, Keisuke;Kishi, Kazuo
上皮细胞和真皮细胞之间的相互作用对于毛囊的形态发生和维持是必不可少的。在毛发再生的实验中，分离的真皮细胞已被证明具有毛发诱导能力。然而，真皮细胞在培养后立即失去这种潜力。来源于真皮的球形多能细胞具有毛发诱导能力。这些先前的发现提出了一个问题，即毛发诱导能力是否取决于真皮细胞的身份或球体形成的过程。为了解决这个问题，我们比较了二维培养或其后球形成诱导的真皮和肺间充质细胞的体外和体内特性。我们发现，球体形成间充质细胞表现出更高的Wnt信号基因的表达。成球细胞而不是二维培养的细胞具有体内毛发诱导能力。这些数据表明，各种间充质细胞通过球体形成过程获得毛发诱导能力。
Interactions between epithelial and dermal cells are essential for hair follicle morphogenesis and maintenance. In experimental trials of hair regeneration, isolated dermal cells have been shown to possess hair-inducing capacity. However, dermal cells lose this potential immediately after cultivation. Sphere-forming multipotent cells derived from the dermis possess hair-inducing capacity. These previous findings raise the question of whether hair-inducing capacity depends on the identity as dermal cells or the process of sphere formation. To address this issue, we compared the in vitro and in vivo characteristics of two-dimensionally cultured or thereafter sphere formation-induced dermal and lung mesenchymal cells. We show that sphere-forming mesenchymal cells exhibited higher expression of Wnt signalling genes. Sphere-forming cells but not two-dimensionally cultured cells possessed in vivo hair-inducing capacity. These data suggest that various mesenchymal cells attain hair-inducing capacity through the process of sphere formation.