PIN and PILS family genes analyses in Chrysanthemum seticuspe reveal their potential functions in flower bud development and drought stress.
PIN and PILS family genes analyses in Chrysanthemum seticuspe reveal their potential functions in flower bud development and drought stress.
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DOI：
10.1016/j.ijbiomac.2022.08.065
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发表时间：
2022-08
影响因子：
8.2
通讯作者：
Lisheng Zhai;Liuhui Yang;Xiangyu Xiao;Jiafu Jiang;Z. Guan;W. Fang;Fadi Chen;Sumei Chen
中科院分区：
文献类型：
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作者：
Lisheng Zhai;Liuhui Yang;Xiangyu Xiao;Jiafu Jiang;Z. Guan;W. Fang;Fadi Chen;Sumei Chen
生长素几乎影响所有的植物生长发育过程。PIN- formed （PIN）和PIN- likes （PILS）家族基因通过在细胞膜上的极性定位决定生长素流动的方向和分布梯度。但是，目前还没有对菊花中pins和pils家族基因进行系统的研究。在菊花中共鉴定到18个pins基因和13个pils基因。分析了这些基因的进化关系、理化性质、保守基序、顺式作用元件、染色体定位、共线性和表达特征。CsPIN10a、CsPIN10b和cspin10care是唯一的epingenes公司的产品。表达模式分析表明，这些基因具有不同的组织特异性，cspin8、CsPINS1、CsPILS6和cspils10的表达水平与花蕾发育时期呈线性相关。原位杂交结果显示，cspin1a、CsPIN1b和cspils8在小花原基和花瓣尖端表达，cspin1a在苞片原基中部特异性表达，可能调控了苞片的横向扩张。cspin和cspils家族基因也参与干旱胁迫反应。本研究为培育花型美观、耐旱性高的品种提供了理论支持。
Auxin affects almost all plant growth and developmental processes. The PIN-FORMED (PIN) and PIN-LIKES (PILS) family genes determine the direction and distribution gradient of auxin flow by polar localization on the cell membrane. However, there are no systematic studies onPINandPILSfamily genes in chrysanthemum. Here, 18PINand 13PILSgenes were identified inChrysanthemum seticuspe. The evolutionary relationships, physicochemical properties, conserved motifs, cis-acting elements, chromosome localization, collinearity, and expression characteristics of these genes were analyzed.CsPIN10a,CsPIN10b,andCsPIN10care uniquePINgenes inC. seticuspe. Expression pattern analysis showed that these genes had different tissue specificities, and the expression levels ofCsPIN8,CsPINS1,CsPILS6,andCsPILS10were linearly related to the developmental period of the flower buds.In situhybridization assay showed thatCsPIN1a,CsPIN1b,andCsPILS8were expressed in floret primordia and petal tips, andCsPIN1awas specifically expressed in the middle of the bract primordia, which might regulate lateral expansion of the bracts.CsPINandCsPILSfamily genes are also involved in drought stress responses. This study provides theoretical support for the cultivation of new varieties with attractive flower forms and high drought tolerance.