A Putative PP2C-Encoding Gene Negatively Regulates ABA Signaling in Populus euphratica.
A Putative PP2C-Encoding Gene Negatively Regulates ABA Signaling in Populus euphratica.
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假定的 PP2C 编码基因负向调控胡杨中的 ABA 信号传导
DOI：
10.1371/journal.pone.0139466
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Tian Q
中科院分区：
文献类型：
--
作者：
Chen J;Zhang D;Zhang C;Xia X;Yin W;Tian Q
从干旱处理的胡杨cDNA文库中克隆了一个PP 2C同源基因。多序列比对分析表明，该基因是HAB 1的潜在直系同源物。该HAB 1直系同源物（PeHAB 1）的表达显著地由干旱诱导，并且适度地由阿坝诱导。为了表征其在阿坝信号传导中的功能，我们产生了过表达该基因的转基因拟南芥植物。与野生型系相比，转基因系对外源阿坝的反应降低，对干旱的耐受性降低。酵母双杂交分析表明，PeHAB 1可以相互作用与阿坝受体PYL 4在ABA-独立的方式。这些结果表明，PeHAB 1是白杨阿坝反应的一个新的负调节因子。
A PP2C homolog gene was cloned from the drought-treated cDNA library of Populus euphratica. Multiple sequence alignment analysis suggested that the gene is a potential ortholog of HAB1. The expression of this HAB1 ortholog (PeHAB1) was markedly induced by drought and moderately induced by ABA. To characterize its function in ABA signaling, we generated transgenic Arabidopsis thaliana plants overexpressing this gene. Transgenic lines exhibited reduced responses to exogenous ABA and reduced tolerance to drought compared to wide-type lines. Yeast two-hybrid analyses indicated that PeHAB1 could interact with the ABA receptor PYL4 in an ABA-independent manner. Taken together; these results indicated that PeHAB1 is a new negative regulator of ABA responses in poplar.