Preparation of agarose/chitosan composite supermacroporous monolithic cryogels for affinity purification of glycoproteins.
Preparation of agarose/chitosan composite supermacroporous monolithic cryogels for affinity purification of glycoproteins.
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DOI：
10.1002/jssc.201100940
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发表时间：
2012-04
影响因子：
3.1
通讯作者：
Sijuan Sun;Yuhai Tang;Qiang Fu;Xuan Liu;Wei Du;Li'an Guo;Yanding Zhao
中科院分区：
文献类型：
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作者：
Sijuan Sun;Yuhai Tang;Qiang Fu;Xuan Liu;Wei Du;Li'an Guo;Yanding Zhao
制备了超大孔琼脂糖/壳聚糖复合整体（AC CM）冻凝胶，用于亲和纯化鸡蛋主要白色糖蛋白卵清蛋白（OVA）和卵转铁蛋白（OVT）。以琼脂糖/壳聚糖共混溶液为原料，戊二醛为交联剂，采用低温共聚合法制备了大孔AC CM冻凝胶。3-氨基苯硼酸配体通过共价键合到环氧基偶联的超大孔AC CM冻凝胶上进行固定。利用扫描电子显微镜对凝胶的微观形貌进行了分析。超大孔AC CM冻凝胶包含连续互穿聚合物网络基质，具有尺寸为10-100 μm的互连孔。该复合冻凝胶具有较高的机械稳定性，并对糖蛋白具有特异性识别。对OVA的最大吸附容量为55.6mg/g。该基质可重复使用11次，而对OVA的吸附能力无明显损失。从鸡蛋白色中回收卵清蛋白和卵清蛋白的回收率分别为89%和93%。
Supermacroporous agarose/chitosan composite monolithic (AC CM) cryogels were prepared for affinity purification of the major egg white glycoproteins, ovalbumin (OVA), and ovotransferrin (OVT). The supermacroporous AC CM cryogels were produced by cryocopolymerization of agarose/chitosan blend solutions using glutaraldehyde as the cross-linker. The 3-aminophenlyboronic acid ligand was immobilized by covalent binding to epoxy-group-coupled supermacroporous AC CM cryogels. The microstructure morphologies of these cryogels were analyzed by scanning electron microscopy. The supermacroporous AC CM cryogels contained a continuous interpenetrating polymer network matrix with interconnected pores of 10-100 μm in size. The composite cryogels offered high mechanical stability and had specific recognition for glycoproteins. The maximum binding capacity of OVA adsorption from aqueous solutions was 55.6 mg/g. The matrix could be reused 11 times without significant loss in OVA adsorption capacity. The recovery yields of OVA and OVT from egg white were estimated to be 89 and 93%, respectively.