Integrated metatranscriptomics and metaproteomics for the characterization of bacterial microbiota in unfed Ixodes ricinus
Integrated metatranscriptomics and metaproteomics for the characterization of bacterial microbiota in unfed Ixodes ricinus
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DOI：
10.1016/j.ttbdis.2018.04.020
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发表时间：
2018-07-01
影响因子：
3.2
通讯作者：
de la Fuente, Jose
中科院分区：
文献类型：
--
作者：
Hernandez-Jarguin, Angelica;Diaz-Sanchez, Sandra;de la Fuente, Jose
一种创新的元组学方法整合元转录组学和元蛋白质组学被用来表征细菌群落的莱姆病螺旋体载体，蓖麻硬蜱（阿卡里：硬蜱科）的微生物群。分别使用未进食幼虫和雌性蜱的整个内部组织和唾液腺。再用岛用于元转录组学分析的蓖麻RNA测序数据与元蛋白质组学一起通过增加细菌鉴定和对具有推定功能意义的鉴定细菌的支持，提供了蜱细菌微生物群的更好表征。结果表明，该菌中存在共生菌、细菌、土壤菌、环境菌和病原菌。蓖麻微生物群，包括以前未被认识的植物和土壤微生物。元组学方法的结果可能对病原体感染操纵蜱微生物群以促进感染的推定机制的表征具有影响。整合不同组学数据集的元组学方法将更好地描述蜱虫微生物群组成，并深入了解蜱虫与微生物群，病原体和宿主的相互作用。
An innovative metaomics approach integrating metatranscriptomics and metaproteomics was used to characterize bacterial communities in the microbiota of the Lyme borreliosis spirochete vector, Ixodes ricinus (Acari: Ixodidae). Whole internal tissues and salivary glands from unfed larvae and female ticks, respectively were used. Reused I. ricinus RNA-sequencing data for metranscriptomics analysis together with metaproteomics provided a better characterization of tick bacterial microbiota by increasing bacteria identification and support for identified bacteria with putative functional implications. The results showed the presence of symbiotic, commensal, soil, environmental, and pathogenic bacteria in the I. ricinus microbiota, including previously unrecognized commensal and soil microorganisms. The results of the metaomics approach may have implications in the characterization of putative mechanisms by which pathogen infection manipulates tick microbiota to facilitate infection. Metaomics approaches integrating different omics datasets would provide a better description of tick microbiota compositions, and insights into tick interactions with microbiota, pathogens and hosts.