Distribution of nitric oxide synthase immunoreactivity in the nervous system and peripheral tissues of Schistosoma mansoni
Distribution of nitric oxide synthase immunoreactivity in the nervous system and peripheral tissues of Schistosoma mansoni
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DOI：
10.1017/s0031182000007022
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发表时间：
2001-01-01
期刊：
影响因子：
2.4
通讯作者：
Greenberg, RM
中科院分区：
文献类型：
--
作者：
Kohn, AB;Moroz, LL;Greenberg, RM
采用3种不同类型一氧化氮合酶（NOS）抗体和NADPH-diaphorase组织化学方法，分析了成年曼氏血吸虫一氧化氮合酶（NOS）免疫反应性的分布和NOS推定活性。虽然已经提出气态自由基一氧化氮（NO）可能参与宿主对血吸虫感染的反应，但关于NO途径在血吸虫本身中的作用，甚至存在的信息很少或没有。在这里，我们证明了针对神经元NOS （nNOS）和诱导型NOS （iNOS）亚型的抗体以不同的模式染色成虫；抗内皮细胞NOS （eNOS）无选择性标记，在主要神经索和周围神经系统中发现nnos样免疫反应。假定的传感器吗?顶端神经元突起导致雄虫被皮的神经元对nNOS也有免疫反应。抗inos标记多种主要是非神经元组织，在蠕虫表面或其附近以及胃肠道成分中显示出强烈的标记。nadph - diaphase反应性（NOS的一种组织化学标记物）的分布与NOS免疫反应性的模式大致相似，包括神经元样细胞和发育中的卵子的标记。这些结果表明，mansoni中存在一种NOS样酶，并提示不同NOS亚型在神经元信号传导、繁殖和发育中的潜在作用。
The distribution of nitric: oxide synthase (NOS) immunoreactivity and putative NOS activity in adult Schistosoma mansoni was analysed using 3 different types of NOS antibodies and NADPH-diaphorase histochemistry. Although potential involvement of the gaseous radical nitric oxide (NO) in host response to infection by schistosomes has been suggested, there is little or no information available regarding the role, or even the presence, of the NO pathway in schistosomes themselves. Here, we demonstrate that antibodies against neuronal NOS (nNOS) and inducible NOS (iNOS) isoforms stain adult worms with distinctive patterns; anti-endothelial NOS (eNOS) shows no selective labelling, nNOS-like immunoreactivity is found ill the main nerve cords and the peripheral nervous system. Putative sensor? neurons with apical neuronal processes leading to the tegument of male worms are also immunoreactive for nNOS. Anti-iNOS labels a variety of predominantly nun-neuronal tissues, showing intense labelling at or near the surface of the worm and in components of the gastrointestinal tract. The distribution of NADPH-diaphorase reactivity (a histochemical marker of NOS), is generally similar to the pattern of NOS immunoreactivity, including labelling of neuronal-like cells as well as developing eggs. These results suggest that an NOS-like enzyme is present in S. mansoni, and indicate potential roles for the different NOS isoforms in neuronal signalling, reproduction and development.