LIPID VESICLES AS CARRIERS FOR INTRODUCING MATERIALS INTO CULTURED-CELLS - INFLUENCE OF VESICLE LIPID-COMPOSITION ON MECHANISM(S) OF VESICLE INCORPORATION INTO CELLS
LIPID VESICLES AS CARRIERS FOR INTRODUCING MATERIALS INTO CULTURED-CELLS - INFLUENCE OF VESICLE LIPID-COMPOSITION ON MECHANISM(S) OF VESICLE INCORPORATION INTO CELLS
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DOI：
10.1073/pnas.73.5.1603
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发表时间：
1976-01-01
影响因子：
11.1
通讯作者：
PAPAHADJOPOULOS, D
中科院分区：
文献类型：
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作者：
POSTE, G;PAPAHADJOPOULOS, D
研究了BALB/c小鼠3T3细胞摄取单层和多层脂质囊泡的机制。囊泡可以通过内吞作用和非内吞机制并入细胞，非内吞机制可能涉及囊泡与质膜的融合。非内吞途径主要是在37度时吸收由液体磷脂（磷脂酰丝氨酸/磷脂酰胆碱）组成的带负电荷的囊泡。中性液体囊泡（磷脂酰胆碱）和由固体磷脂（磷脂酰丝氨酸/二硬脂酰磷脂酰胆碱/二棕榈酰磷脂酰胆碱）制备的带负电荷的囊泡主要通过内吞作用被吸收。后一类囊泡的摄取被细胞能量代谢抑制剂和细胞松弛素b减少（抑制80-90%）。在类似处理后，带负电荷的液体囊泡的摄取仅减少30-40%。用戊二醛预先固定细胞可抑制内吞作用和中性和带负电荷的固体囊泡的摄取，但不抑制带液体的囊泡的摄取。用OsO4预先固定细胞可阻止所有类型的囊泡的摄取。相同组成的单层和多层小泡也得到了类似的结果，表明小泡组成比大小在决定细胞摄取途径方面更重要。讨论了囊泡摄取的不同途径，并讨论了将囊泡靶向到特定细胞内位置的可能方法的发展。
The mechanisms involved in the uptake of uni- and multi-lamellar lipid vesicles by BALB/c mouse 3T3 cells were investigated. Vesicles are incorporated into cells both by endocytosis and by a nonendocytotic mechanism which probably involves fusion of vesicles with the plasma membrane. The nonendocytotic pathway predominates in the uptake of negatively charged vesicles composed of phospholipids that are fluid (phosphatidylserine/phosphatidylcholine) at 37.degree.. Neutral fluid vesicles (phosphatidylcholine) and negatively charged vesicles prepared from solid phospholipids (phosphatidylserine/distearylphosphatidylcholine/dipalmitoylphosphatidylcholine) are instead incorporated largely by endocytosis. Uptake of the latter classes of vesicle is reduced (80-90% inhibition) by inhibitors of cellular energy metabolism and by cytochalasin B. Uptake of negatively charged fluid vesicles is reduced by only 30-40% after similar treatments. Prefixation of cells with glutaraldehyde inhibits endocytosis and uptake of neutral and negatively charged solid vesicles, but does not inhibit uptake of fluid charged vesicles. Prefixation of cells with OsO4 prevents uptake of all classes of vesicles. Similar results are obtained with both uni- and multi-lamellar vesicles of the same composition, indicating that vesicle composition rather than size is more important in determining the pathway of uptake into cells. The different pathways involved in vesicle uptake are discussed in relation to the development of possible methods for targeting vesicles to specific intracellular sites.