Human adipose tissue-derived stromal/stem cells promote migration and early metastasis of triple negative breast cancer xenografts.
Human adipose tissue-derived stromal/stem cells promote migration and early metastasis of triple negative breast cancer xenografts.
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DOI：
10.1371/journal.pone.0089595
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Chiu ES
中科院分区：
文献类型：
--
作者：
Rowan BG;Gimble JM;Sheng M;Anbalagan M;Jones RK;Frazier TP;Asher M;Lacayo EA;Friedlander PL;Kutner R;Chiu ES
脂肪移植用于修复乳房肿瘤手术切除后的乳房缺损。建议用脂肪组织来源的基质/干细胞（ASC）补充脂肪移植物以改善移植物的再生/恢复能力和保留。然而，ASC移植在残留乳腺癌细胞附近的长期安全性尚不清楚。本研究的目的是确定来自三个供体腹部脂肪抽吸物的人ASC对表现出早期转移的人乳腺癌模型的影响。人MDA-MB-231乳腺癌细胞代表表现出向多个小鼠器官的早期微转移的“三阴性”乳腺癌。人ASC来源于来自三个健康女性供体的腹部脂肪组织。MDA-MB-231细胞与ASC的间接共培养以及直接共培养证明ASC对MDA-MB-231生长没有影响。间接共培养和ASC条件培养基（CM）刺激MDA-MB-231细胞的迁移。来自与MDA-MB-231/GFP细胞共注射的两个供体的ASC/RFP细胞表现出刺激原发性肿瘤异种移植物的供体效应。两个ASC供体均刺激转移。ASC/RFP细胞是活的，并且与肿瘤中的MDA-MB-231/GFP细胞整合。来自一个ASC供体的共注射组的肿瘤表现出升高的波形蛋白、基质金属蛋白酶-9（MMP-9）、IL-8、VEGF和微血管密度。共注射组表现出可见的肺/肝转移和脾肿大，在单独注射MDA-MB-231/GFP的小鼠中不明显。小鼠器官中GFP荧光和人染色体17 DNA的总面积的定量、H&E染色的石蜡切片和荧光显微镜检查证实了共注射组中肺/肝/脾的多灶性转移，而没有ASC/RFP细胞的证据。来源于两个供体的腹部脂肪抽吸物的人ASC刺激MDA-MB-231乳腺肿瘤异种移植物转移到多个小鼠器官。与来自一个供体的ASC共注射的MDA-MB-231肿瘤表现出部分EMT、MMP-9表达和增加的血管生成。
Fat grafting is used to restore breast defects after surgical resection of breast tumors. Supplementing fat grafts with adipose tissue-derived stromal/stem cells (ASCs) is proposed to improve the regenerative/restorative ability of the graft and retention. However, long term safety for ASC grafting in proximity of residual breast cancer cells is unknown. The objective of this study was to determine the impact of human ASCs derived from abdominal lipoaspirates of three donors, on a human breast cancer model that exhibits early metastasis. Human MDA-MB-231 breast cancer cells represents “triple negative” breast cancer that exhibits early micrometastasis to multiple mouse organs. Human ASCs were derived from abdominal adipose tissue from three healthy female donors. Indirect co-culture of MDA-MB-231 cells with ASCs, as well as direct co-culture demonstrated that ASCs had no effect on MDA-MB-231 growth. Indirect co-culture, and ASC conditioned medium (CM) stimulated migration of MDA-MB-231 cells. ASC/RFP cells from two donors co-injected with MDA-MB-231/GFP cells exhibited a donor effect for stimulation of primary tumor xenografts. Both ASC donors stimulated metastasis. ASC/RFP cells were viable, and integrated with MDA-MB-231/GFP cells in the tumor. Tumors from the co-injection group of one ASC donor exhibited elevated vimentin, matrix metalloproteinase-9 (MMP-9), IL-8, VEGF and microvessel density. The co-injection group exhibited visible metastases to the lung/liver and enlarged spleen not evident in mice injected with MDA-MB-231/GFP alone. Quantitation of the total area of GFP fluorescence and human chromosome 17 DNA in mouse organs, H&E stained paraffin sections and fluorescent microscopy confirmed multi-focal metastases to lung/liver/spleen in the co-injection group without evidence of ASC/RFP cells. Human ASCs derived from abdominal lipoaspirates of two donors stimulated metastasis of MDA-MB-231 breast tumor xenografts to multiple mouse organs. MDA-MB-231 tumors co-injected with ASCs from one donor exhibited partial EMT, expression of MMP-9, and increased angiogenesis.
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影响因子：
2.4
作者：
FOURNIER, PF
通讯作者：
FOURNIER, PF
影响因子：
2.9
作者：
Delay, Emmanuel;Garson, Sebastian;Sinna, Raphael
通讯作者：
Sinna, Raphael
DOI：
10.1055/s-2004-861755
发表时间：
2004-05-01
期刊：
Facial plastic surgery : FPS
影响因子：
--
作者：
Cook, Todd;Nakra, Tanuj;Douglas, Raymond S
通讯作者：
Douglas, Raymond S
影响因子：
1.5
作者：
Hu, Shouduo;Zhang, Haiming;Shi, Jun
通讯作者：
Shi, Jun
影响因子：
3.8
作者：
Chandler, Emily M.;Berglund, Caroline M.;Fischbach, Claudia
通讯作者：
Fischbach, Claudia