Ingi, a 5.2-kb dispersed sequence element from Trypanosoma brucei that carries half of a smaller mobile element at either end and has homology with mammalian LINEs
Ingi, a 5.2-kb dispersed sequence element from Trypanosoma brucei that carries half of a smaller mobile element at either end and has homology with mammalian LINEs
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DOI：
10.1128/mcb.7.4.1465-1475.1987
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发表时间：
1987-04
影响因子：
5.3
通讯作者：
Bruce E. Kimmel;K. Onesmo;OLE-MOIYOI;John R. Young
中科院分区：
文献类型：
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作者：
Bruce E. Kimmel;K. Onesmo;OLE-MOIYOI;John R. Young
本文描述了一种存在于原生动物寄生虫布氏锥虫基因组中的分散重复元件，命名为ingi。对一个完整的5.2-内切酶元件和另外两个元件的末端进行了测序。没有直接或反向末端重复序列。相反，末端由先前描述的512个碱基对的转座因子的两半组成（G。Hasan，M.J. Turner和J.S. Cordingley，Cell 37：333-341，1984）。寡核苷酸（dA）尾巴和可能的插入位点重复表明ingi是一个逆转录酶。测序元件似乎是原始逆转录子的假基因拷贝，其中一个或多个开放阅读框占据了其大部分长度。编码的氨基酸序列与逆转录酶和哺乳动物长间隔核元件序列的显着同源性表明这种逆转录子的远程进化起源。
A dispersed repetitive element named ingi, which is present in the genome of the protozoan parasite Trypanosoma brucei, is described. One complete 5.2-kilobase element and the ends of two others were sequenced. There were no direct or inverted terminal repeats. Rather, the ends consisted of two halves of a previously described 512-base-pair transposable element (G. Hasan, M.J. Turner, and J.S. Cordingley, Cell 37:333-341, 1984). Oligo(dA) tails and possible insertion site duplications suggested that ingi is a retroposon. The sequenced element appears to be a pseudogene copy of an original retroposon with one or more open reading frames occupying most of its length. Significant homologies of the encoded amino acid sequences with reverse transcriptases and mammalian long interpersed nuclear element sequences suggest a remote evolutionary origin for this kind of retroposon.