MURINE T-CELL CLONES SPECIFIC FOR CHICKEN ERYTHROCYTE ALLOANTIGENS
MURINE T-CELL CLONES SPECIFIC FOR CHICKEN ERYTHROCYTE ALLOANTIGENS
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DOI：
10.1016/0008-8749(86)90406-5
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发表时间：
1986-02-01
影响因子：
4.3
通讯作者：
MOSMANN, TR
中科院分区：
文献类型：
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作者：
GIEDLIN, MA;LONGENECKER, BM;MOSMANN, TR
我们已经建立了对鸡红细胞(CRBC)上发现的异型和物种特异性决定簇做出反应的小鼠T细胞克隆。通过检测主要组织相容性复合体(MHC)抗原纯合子鸡的同基因脾细胞和CRBC产生的淋巴因子和增殖反应来确定它们的相对抗原特异性。这种特异性模式表明，与产生抗体的细胞相比，T细胞克隆识别的CRBC MHC相关同种决定簇更加有限。抗原特异性反应需要抗原处理，并受到MHC的限制和抗原剂量的依赖。大约20%来自适当品系的小鼠的T细胞克隆也是MLS同种异体反应的。第二种反应性与标称CRBC特异性无关。T细胞生长因子、肥大细胞生长因子和Ia诱导因子的诱导特异性淋巴因子活性被鉴定为白介素2(IL-2)、白介素3(IL-3)和干扰素-γ。分别进行了分析。
We have established murine T-cell clones which respond to allotypic and species-specific determinants found on chicken erythrocytes (cRBC). Their relative antigen specificities were determined by assessing lymphokine production and proliferation in response to syngeneic spleen cells and cRBC obtained from chickens homozygous for major histocompatibility complex (MHC) antigens. The specificity pattern suggested that the T-cell clones recognized a more restricted set of cRBC MHC-associated allodeterminants than do antibody-producing cells. The antigen-specific responses required antigen processing, and were MHC restricted and antigen dose dependent. Approximately 20% of T-cell clones from appropriate strains of mice were also Mls alloreactive. This second reactivity showed no correlation with nominal cRBC specificity. The induction-specific lymphokine activities of T-cell growth factor, mast cell growth factor, and Ia induction factor were identified as interleukin 2 (IL-2), interleukin 3 (IL-3), and interferon-.gamma. respectively.