Spurious splicing within the XIAP 5′ UTR occurs in the Rluc/Fluc but not the βgal/CAT bicistronic reporter system
Spurious splicing within the XIAP 5′ UTR occurs in the Rluc/Fluc but not the βgal/CAT bicistronic reporter system
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作者：
Holcik, M;Graber, T;Baird, S
x染色体连接的凋亡抑制剂XIAP，已被证明在其5'非翻译区（UTR）中含有一个强大的内部核糖体进入位点（IRES），在细胞应激条件下促进XIAP mRNA的翻译。这一说法在最近的一份报告中受到了仔细的审查，该报告表明，当插入到双荧光素酶质粒Rluc/Fluc的两个报告子之间时，XIAP 5' UTR会发生剪接。在本文中，我们证明了XIAP 5' UTR内的剪接仅发生在Rluc/Fluc产生的mRNA中，而不发生在p β gal/CAT双子报告质粒中。
X-chromosome-linked inhibitor of apoptosis, XIAP, has been shown to contain a strong internal ribosome entry site (IRES) within its 5' untranslated region (UTR) that promotes translation of XIAP mRNA under conditions of cellular stress. This claim came under scrutiny in a recent report demonstrating that the XIAP 5' UTR undergoes splicing when inserted between the two reporter cistrons of the dual luciferase plasmid Rluc/Fluc. in this paper, we demonstrate that the splicing within the XIAP 5' UTR specifically occurs only in the context of mRNA produced from the Rluc/Fluc but not the p beta gal/CAT bicistronic reporter plasmid.